中文摘要：

目的：研究缺血再灌注（ischemia／reperfusion，I／R）对大鼠骨骼肌组织蛋白质表达谱的影响。方法：健康雄性Wistar大鼠12只随机分为2组（n=6）：假手术组和I／R组，无创动脉夹夹闭右侧股动脉4h，松夹再灌注24h建立I／R模型；实验结束时提取骨骼肌组织蛋白质，双向电泳技术分离骨骼肌组织蛋白质，分析差异显示的蛋白质并选取7个差异显著的蛋白点进行质谱分析。结果：双向电泳可分离（354±13）个蛋白质，点匹配率为（78．7±1．4）％，VR诱导骨骼肌组织10种蛋白质出现明显差异表达，其中6种表达上调，3种表达下调，1种在I／R组为2个点。质谱鉴定出5个蛋白质为：线粒体醛脱氢酶（mitochondrial Mdehyde dehydrogenase，ALDH）前体、热休克蛋白27（heat shock 27 kD protein，HSP27）和一未命名蛋白（I／R组表达升高）、α-肌动蛋白（α-actin，I／R组表达下降）、核转移因子2（nuclear transport factor 2，NTF-2）在I／R组发生突变。结论：I／R损伤引起大鼠骨骼肌蛋白质表达发生改变，其中α-肌动蛋白、ALDH和HSP27表达及NTF-2突变可能与I／R损伤有关。

英文摘要：

AIM： To investigate ischemia reperfusion （I/R） -induced proteomic changes in rat skeletal muscle. METHODS： Healthy male Wistar rats were randomly divided into two groups as follows （n = 6） ： sham group and I/R group. I/R of fight hind limb was induced by 4 h ischemia followed by 24 h reperfusion. The 2 - DE was applied to separate the proteins extracted from skeletal muscle tissue at the end of experiment, followed by Coomassie Brillant blue R- 250 staining. Computer image analysis was used to determine the differential expression of proteins between the two groups, and 7 protein spots expressed differentially were picked out and subjected to in - gel digest and MALDI - TOP for identification. RESULTS： 354±13 proteins were detected and the match rate was （78.7±1.4）%. 10 proteins displayed significant changes after I/R, of which, 6 proteins increased and 3 proteins decreased in expression. Moreover, 2 spots in I/R group were observed, only 1 spots of which in control. 5 proteins were identified after mass spectrometry. Mitochondrial aldehyde dehydrogenase （ALDH） precursor, heat shock 27 kD protein （HSP27）, an unnamed protein product （increased in I/R group）, α -actin （decreased in I/R group）, and nuclear transport factor 2 （NTF -2） W7a mutant were found in I/R group. CONCLUSION： I/R injury induced differential proteomic changes in rat skeletal muscle. ALDH, α -actin and HSP27 expression, and NTF- 2 mutation are involved in I/R injury.