中文摘要：

目的：建立神经导向研究的模型，并鉴定netrin-l的神经导向功能。方法：用促性腺素释放激素（Gonadotropin-releasing hormone，GnRH）神经细胞为研究模型，建立双细胞团培养方法，用netrin-1诱导GnRH神经元亚克隆系NLT，观察细胞生长的诱导情况。结果：NLT细胞团在体外培养条件下，具有自发放射状迁移能力。在netrin-1诱导作用下，NLT细胞朝向netrin-1浓度梯度的方向生长，在靠近netrin-1细胞团的部位，netrin-1浓度梯度高，迁移出的细胞数目较多，约占总数目的68％，并且细胞迁移的平均距离较长为107-31μm；而在远离netrin-1细胞团部位，其netrin-1浓度梯度较低，迁移出的细胞数目相对较少约占32％，细胞迁移的平均距离较短为56．52μm。结论：netrin-1能诱导培养的NLT细胞发生明显的定向迁移，这为进一步深入研究netrin-1在神经系统中的功能和分子机制奠定了基础。

英文摘要：

Objective： To set up a cell model for axon guidance and identify the function of netrin-1 in axon guidance. Method： Gonadotropin-releasing hormone （GnRH） neuron, which has the ability of migration in Vitro, was used. A system of double cell aggregates was set up to investigate the role of netrin-1 in migration of GnRH neuron. Results： NLT cells migrated radiatedly and spontaneously in Vitro from cultured cell aggregate. The NLT cells induced by netrin-1 migrated forward the higher netrin-1 concentration. The higher concentration, the more cell migration. We found that 68 % of NLT migrated into the proximal quadrant of the netrin-1 cell aggregate and 32 % of NLT migrated into distal quadrant; the migrated distance in the proximal quadrant and distal quadrant were 107.31 μm and 56.52μm, respectively. Conclusions： Migration of GnRH neurons was guided by netrin-1 in Vitro. This model will be important for further study on functions and molecular mechanisms ofnetrin-1 in the nervous system.