中文摘要：

目的观察消癌解毒方对人肝癌SMMC-7721细胞相关趋化因子蛋白表达的影响，探讨消癌解毒方抗肿瘤的作用机制。方法消癌解毒方作用于人肝癌SMMC-7721细胞8h后采用蛋白芯片技术检测药物作用前后各组相关趋化因子蛋白表达水平，采用Westernblot法检测用药后各组单核细胞趋化蛋白3（MCP-3）蛋白的表达。结果与模型组比较，共有9个趋化因子蛋白下调，其中，消癌解毒方4mg·mL-1组有3个蛋白表达下调，分别是MCP-3、Eotaxinl、ENA78；消癌解毒方2mg·mL-1组有3个蛋白表达下调，分别是MCP-3、CCL28、BLC；顺铂组共有6个蛋白表达下调，分别是CXCL16、ENA78、Eotaxin1、XCLl、MIP-3beta、MPIF1。Westernblot结果显示，与模型组比较，消癌解毒方4mg·mL-1组、2mg·mL-1组McP-3蛋白均出现下调，顺铂组无明显差异。结论消癌解毒方可能是通过影响趋化因子信号通路中的某些蛋白来发挥抗肿瘤的作用。

英文摘要：

Objective To observe the effects of Xiao~d Jiedu Prescription（XJP） on protein expression of chemokines related to human hepatoeellular carcinoma SMMC-7721 ceils, and to explore its anti-tumor mechanism. Methods The protein chip technique was used to examine the protein expression of related chemokines in all of the groups after treatment of human hepatocellular carcinoma SMMC-7721 cells with XJP for 8 hours. The Western blotting was used to detect the expression of MCP -3 in each group after treatment. Results Compared with the model control group, the protein expression in a total of 9 chemokines was down-regulated in XJP groups and cis-diamminediehloroplatinum （DDP） group. The downregulated proteins were MCP-3, Eotaxin 1 and ENA 78 in 4 mg·mL-1 XJP group, MCP-3, CCL28 and BLC in 2 mg＇mL-1XJP group, and CXCL16, ENA 78, Eotaxin 1, XCL1, MIP-3 beta and MPIF 1 in the DDP group. Western blotting results revealed that MCP -3 was down-regulated in 4 mg·mL-1 XJP group and 2 mg·mL-1 XJP group compared with the model group, while DDP group showed no significant difference. Conclusion The anti-tumor mechanism of XJP is probably associated with affecting certain proteins in signaling pathways of chemokines.