中文摘要：

支持因素 E (RpfE ) 的复活是在 Mycobacterium 肺结核的五象 Rpf 一样蛋白质之一(M。肺结核) 。这些象 Rpf 一样蛋白质是能分泌的，它由主人免疫系统为识别使他们成为候选人。在这研究， RpfE 基因从 M 被放大。肺结核，克隆进表示向量 pDE22 和 pPRO EXHT，并且在 Mycobacterium vaccae 被表示(M。vaccae ) 并且 Escherichia coli DH5 分别地。recombinant RpfE 蛋白质被 Ni-Sepharose 亲密关系层析净化，并且被给 C57BL/6 老鼠。RpfE 蛋白质得到了 T 房间增长，并且刺激了 gamma 干扰素(IFN-) 的生产， interleukin-10 (IL-10 ) 和 IL-12。我们的结果显示 RpfE 蛋白质在 M 表示了。vaccae 能更高效地刺激细胞的有免疫力的反应，使它成为象一支子单元疫苗的一个有希望的候选人。

英文摘要：

Resuscitation promoting factor E （RpfE） is one of the five Rpf-like proteins in Mycobacterium tuberculos＆ （M. tuberculosis）. These Rpf-like proteins are secretory, which make them candidates for recognition by the host immune system. In this study, the RpfE gene was amplified from M. tuberculosis, cloned into the expression vectors pDE22 and pPRO EXHT, and were expressed in Mycobacterium vaccae （M. vaccae） and Escherichia coli DHSa, respec- tively. Both recombinant RpfE proteins were purified by Ni-Sepharose affinity chromatography, and were given to C57BL/6 mice. The RpfE proteins elicited T cell proliferation, and stimulated the production of gamma interferon （IFN-y）, interleukin-10 （IL-10） and IL-12. Our results indicated that the RpfE protein expressed in M. vaccae could more efficiently stimulate cellular immune response, making it a promising candidate as a subunit vaccine.