中文摘要：

目的观察氧化应激相关指标在模拟急进高原缺氧大鼠肺组织的变化情况。方法成年健康雄性Wistar大鼠40只,随机分为正常对照组、缺氧1、3、5 d组,每组10只。观察缺氧不同时间大鼠肺组织病理学的改变情况,检测肺组织相关生化指标水平。结果缺氧各组大鼠肺组织均有明显损伤,且以缺氧3 d组大鼠损伤尤为严重。与正常对照组比较,缺氧各组大鼠肺组织总抗氧化能力（total antioxidant capacity,TAOC）、还原型谷胱甘肽（reduced glutathione,GSH）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-PX）、总一氧化氮合酶（total nitric oxide synthase,TNOS）和乳酸脱氢酶（lactate dehydrogenase,LDH）在缺氧3 d组显著降低（P〈0.05,P〈0.01）,丙二醛（maleic dialdehyde,MDA）、乳酸（lactate,LD）含量显著升高（P〈0.01）,且随缺氧时间的延长逐渐增加;总超氧化物歧化酶（total superoxide dismutase,TSOD）、Na^＋K^＋-ATP酶活力显著降低（P〈0.05,P〈0.01）,一氧化氮（nitric oxide,NO）含量在缺氧1、3 d组明显降低（P〈0.01）。结论急进高原缺氧大鼠肺损伤机制与氧化应激反应有关,机体抗氧化能力降低、自由基增加是导致肺组织损伤的重要因素,且损伤程度与缺氧暴露时间有关。

英文摘要：

Objective To observe changes of related indexes of oxidative stress in lung tissues of rats under simula-ted acute depression and hypoxia environment. Methods A total of 40 adult male Wistar rats were randomly divided into con-trol group and 1, 3 and 5 d hypoxia groups （n=10 for each group）. Pathological changes of lung tissues at different exposure times were observed, and related indexes of lung tissues were detected in rats. Results Lung tissues of all hypoxia groups were obviously damaged, and the significant damage was found in 3 d hypoxia group. Compared with those in control group, in hypoxia groups, levels of total antioxidant capacity （ TAOC） , reduced glutathione （ GSH） , glutathione peroxidase （ GSH-PX） , total nitric oxide synthase （TNOS） and lactate dehydrogenase （LDH） were decreased, and the most decreased levels were found in 3 d hypoxia group （P〈0. 05, P〈0. 01）;contents of maleic dialdehyde （MDA） and lactic acid （LD） were signifi-cantly increased （P〈0. 01）, and the contents were gradually increased with prolonged hypoxia time;activities of total super-oxide dismutase （TSOD） and Na＋K＋-ATP enzyme were significantly decreased （P〈0. 05, P〈0. 01）;contents of nitric oxide （NO） were significantly decreased in 1 and 3 d hypoxia groups （P〈0. 01）. Conclusion Mechanisms of damage in lung tis-sues of rats relate to oxidative stress reaction under simulated acute depression and hypoxia environment. The decreasing an-tioxidant capacity of the body and increasing free radicals are the important factors leading to damage of lung tissues, and de-gree of lung damage relates to exposure time in depression and hypoxia environment.