采用Roper Scientific SP-2558多功能光谱测量系统对色谱纯甲醇溶液在紫外光激励下产生的荧光光谱进行了实验研究。实验结果表明,甲醇溶液能吸收紫外光并向外发射峰值位于340nm附近的荧光光谱,同时得到了能产生荧光光谱的最长激励光波长的临界值为245nm左右。对甲醇分子发射荧光的机理进行了讨论,给出了与实验结果相一致的理论分析。研究甲醇的荧光光谱可为其作为有机溶剂和催化剂时对其他有机大分子相关光谱的影响提供理论和实验上的参考,并可直接为荧光探针技术的发展提供服务。
Using the Roper Scientific SP-2558 multifunctional spectrometer system, the fluorescent spectra was researched with experiments induced by UV-light, The research indicates that methanol molecules can absorb the UV-light and they can emit fluorescence with the peak value about 340nm, And the critical value of largest exciting wavelength (245nm) was got. The emitting mechanism of methanol molecule was discussed. The investigation on the fluorescence spectra and their characteristic will contribute to study other organic maeromolecule fluorescence spectra when methanol serves as solute and hydrolysis catalyst. The results can be directly used to technology of fluorescent probe.