中文摘要：

目的检测DNA修复酶hOGG1及PARP在原发性肝癌及肝硬化组织中的表达，探讨hOGG1和PARP在HCC和肝硬化组织中的表达特点及其与临床病理的关系。方法应用EnVision免疫组化法检测41例非HCC正常肝组织、99例HCC组织、51例HCC癌旁肝硬化组织中hOGG1和PARP表达情况。结果在HCC组织中hOGG1和PARP在细胞核与细胞质中均有表达，其中hOGG1细胞核阳性分度在正常组、肝硬化组、HCC组之间依次增高（P〈0．001）；PARP细胞核阳性分度在正常组、肝硬化组、HCC组之间依次降低（P〈0．001）。多因素分析显示：hOGG1蛋白在细胞核表达与ALT有关（相对危险度为1．022，P=0.041），hOGG1蛋白在细胞浆表达与总胆红素有关（相对危险度为1．155，P=0．018），PARP蛋白在细胞浆表达与外周血白细胞数（相对危险度为0．757，P=0．037）有关。结论在HCC发生过程中hOGG1蛋白在肝细胞核中的表达可能随着病程进展而增强，PARP蛋白在肝细胞核中的表达可能随着病程进展而降低，可能反应了从肝硬化到肝癌进程中肝细胞内氧化应激状态的改变。

英文摘要：

Objective To explore the expression and clinicopathological implications of human 8-oxoGuanine DNA glycosylase-1 （hOGG1） and poly ADP-ribose polymerase （PARP） in hepatocellular carcinoma （HCC） and hepatic cirrhotic tissues. Methods hOGG1 and PARP expression was detected by immuneohistochemi, EnVision method on liver tissues of non-HCC controls （n =41 ） ,hepatic cirrhotic （n = 51） and HCC patients （n=99）.Results 32aere were three types of hOGG1 and of PARP positive staining in HCCs： nuclear,cytoplasmic and mixed. The expression levels of hOGG1 in nucleus was significantly higher in HCCs than that in hepatocirrhosis and controls,and significantly higher in hepatocirrhosis than that in controls （P 〈0. 01 ）. The expression levels of PARP in nucleus was significantly lower in HCCs than that in hepatocirrhesis and controls,and significantly lower in hepatocirrhosis than that in controls （P 〈0. 01 ）. The expression of hOGG1 and of PARP was not associated with the clinicopathological factors in HCCs （P 〉0.05）. Multiplicity indicated that hOGG1 expression in nucleus was related to ALT （OR = 1.022,P =0.041） ,hOGG1 expression in cytoplasm was related to T bili （OR = 1.155 ,P =0.018 ） ,PARP expression in cytoplasm was related to peripheral blood leucocytes（OR = 0. 757 ,P =0.037）. Conclusion During hepatocarcinogenesis,the expression pattern of hOGG1 and PARP in liver cell nucleus might reflect the status of oxidative stress in each stage thus has etiology implieations.