中文摘要：

目的探讨多指标联合分析多结节肝癌细胞克隆来源的价值。方法选择44例多结节肝癌共116个结节肝癌组织,以P53、AFP蛋白,P53exon7、bcl-10基因,线粒体DNA（mtDNA）D-Loop区序列等5项指标作为鉴别细胞克隆起源异同的指标,判别多结节肝癌各癌结节的克隆起源是否相同。结果经5项指标检测,联合判断为多中心性克隆起源（MO）者22例,单中心性克隆起源（IM）者22例。结论联合检测多结节肝癌组织中P53蛋白、AFP蛋白,P53exon7、BCL-10基因及线粒体DNAD-Loop区序列在各癌结节间的表达差异,有助于鉴别其细胞克隆来源。

英文摘要：

【Objective】To evaluate possibility of characterizing the clonal origin of multinodular hepatocellular carcinoma （HCC） by multi-biomarkers.【Methods】Study group （multinodular HCCs） were 44 patients with a total of 116 HCC nodules.A combination of 5 biomarkers （P53 and AFP proteins,P53 （exon7） and bcl-10 genes mutations,mtDNA D-Loop region variations was applied to characterize the clonal origin of multinodular HCC.【Results】Using biomarker profiling,22 cases were categorized as multicentric occurrence （MO）,and 22 cases were characterized as intrahepatic metastasis （IM）.【Conclusion】Multi-biomarkers approach might identify the clonal origin of multinodular HCC thus help clinical decision-making in practice.