中文摘要：

目的：比较银屑病患者与正常人皮肤间充质干细胞（skin—derivedmesenchymalstemcells，SMSCs）生长特性及其分泌表皮生长因子（EGF）、转化生长因子（TGF）-β1水平，揭示银屑病患者皮损微环境中SMSCs存在异常。方法：酶消化法分离银屑病组与正常人对照组SMSCs，在倒置相差显微镜下观察细胞形态，流式细胞仪检测细胞免疫表型，成脂、成骨诱导体系鉴定细胞多系分化能力，ELISA法检测细胞培养上清液中EGF、TGF-β1浓度。结果：两组细胞形态均存在异质性。第3代SMSCs表面抗原CD29、CD44、CD73、CD90及CDl05表达阳性，CD34、CD45及人白细胞DR抗原（HLA—DR）表达阴性。细胞成脂诱导14d油红O染色阳性，成骨诱导21d茜素红S染色阳性。银屑病组SMSCs分泌EGF水平高于正常人对照组（P〈0．05），分泌TGF-β1水平低于正常人对照组（P〈0．05）。结论：该实验建立了稳定的银屑病患者SMSCs体外分离培养方法，发现细胞形态存在异质性，银屑病组SMSCs分泌EGF、TGF-β1水平异常，提示其皮损微环境中SMSCs可能存在异常。

英文摘要：

Objective： To reveal the abnormality of skin-derived mesenchymal stem cells （SMSCs） in psoriatic lesions microenvi- ronment by comparing the growth properties and the secretion levels of EGF and TGF-β1 in patients and normal control. Meth- ods： SMSCs were isolated and cultured by enzyme digestion from psoriatic lesions and normal control. The cell morphology was observed under the inverted phase contrast microscope. The cell immunophenotypes were analyzed by flow cytometry. The muhilin- cage differentiation potential of SMSCs were identified by adipogenic and osteogenic induction system. The level of EGF and TGF- 131 in medium supernatant were detected by ELISA kits. Results： SMSCs morphology heterogeneity was observed in both psoriatic lesions and normal control. The third passage cells were positive for CD29, CD44, CD73, CD90 and CD105, while were negative for CD34, CD45 and HLA-DR. The cells were induced with adipoeyte differentiation medium for 14 days, Oil Red O staining was positive. The cells were induced with osteoblast medium for 21 days , Alizarin Red S stain was positive. The level of EGF in pa- tients was higher than that in control （P〈0.05）, and the level of TGF-β1 in patients was lower than that in control （P〈0.05）. Conclusions： Stable method of isolation and culture of SMSCs from psoriatic lesions was established in our study, SMSCs mor- phology heterogeneity was observed. The levels of EGF and TGF-β1 secreted by psoriatic SMSCs were detected abnormally, which shows there maybe some abnormal ehan~es exist in SMSCs that located in Dsoriatic lesions microenviromnent.