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拮抗菌X23发酵液对金黄色葡萄球菌的抑制作用
  • ISSN号:1007-1032
  • 期刊名称:湖南农业大学学报(自然科学版)
  • 时间:2012
  • 页码:497-500
  • 分类:R284.2[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]湖南中医药大学,湖南长沙410208, [2]湖南大三湘油茶科技有限公司,湖南衡阳421000
  • 相关基金:国家自然科学基金项目(81072751;81473468); 湖南省自然科学基金项目(13JJ6062); 湖南省教育厅重点项目(13A066); 湖南省高校科技创新团队资助项目(15); 湖南中医药大学重点学科资助项目(1)
  • 相关项目:广谱生防菌X23非核糖体抗菌肽结构及生物合成途径研究
中文摘要:

目的:比较研究油茶粕与油茶粕中药复方的抗菌作用和去垢作用。方法:1采用牛津杯法检测含生药质量浓度为0.5 kg·L(-1)的油茶粕和油茶粕中药复方提取液对伤寒沙门菌、大肠埃希菌、表皮葡萄球菌、金黄色葡萄球菌、铜绿假单胞菌、痢疾杆菌和乙型溶血性链球菌抑菌环直径;同时采用试管稀释法检测其对以上各供试菌的最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimal bactericidal conceutration,MBC);2采用国标GB9985—2000方法测定含生药质量浓度分别为0.117 kg·L(-1)、0.235 kg·L(-1)、0.47 kg·L(-1)的油茶粕和油茶粕中药复方提取液对不锈钢片上污垢的去垢能力。结果:1含生药质量浓度为0.5 kg·L(-1)的油茶粕中药复方提取液对金黄色葡萄球菌、乙型溶血性链球菌的抑菌环直径明显大于相应浓度油茶粕提取液;油茶粕中药复方提取液对伤寒沙门菌、表皮葡萄球菌、金黄色葡萄球菌、痢疾杆菌、乙型溶血性链球菌的MIC值均低于油茶粕,对表皮葡萄球菌、金黄色葡萄球菌、乙型溶血性链球菌的MBC值均低于油茶粕;2油茶粕中药复方提取液的去垢能力明显高于相应浓度油茶粕提取液,以含生药质量浓度为0.117 kg·L(-1)时的差异最明显。结论:油茶粕中药复方对伤寒沙门菌、表皮葡萄球菌、金黄色葡萄球菌、乙型溶血性链球菌的抑菌与和杀菌作用优于油茶粕;油茶粕中药复方的去垢能力明显优于油茶粕。

英文摘要:

Objective: To study the detergent and antibacterial effect of Camellia oleifera meal and related traditional Chinese medicine prescription. Methods: 1 Oxford cup method was applied to detect the concentration of 0. 5 kg·L~(-1)crude drug of Camellia oleifera meal and related Chinese medicine prescription extract to know the inhibitory zone diameters of Salmonella typhi,Escherichia coli,Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa bacteria,Shigella and beta-hemolytic streptococcus. The minimum inhibitory concentration( MIC) and minimum bactericidal concentration( MBC) were also detected by using tube dilution method; 2 By using GB GB9985-2000 method the detergent power of crude drug of Camellia oleifera meal and related Chinese medicine prescription extract in concentrations of 0. 117 kg·L~(-1)crude drug,0. 235 kg·L~(-1)crude drug,0. 47 kg·L~(-1)crude drug of the both were determined. Results: 1 the inhibitory zone diameters of traditional Chinese medicine prescription extracts with concentration of 0. 5 kg·L~(-1)crude drug working on Staphylococcus aureus and beta-hemolytic streptococcus were significantly larger than that of Camellia oleifera meal in the corresponding concentration. The MIC values of the extracts on Salmonella typhi,Staphylococcus epidermidis,Staphylococcus aureus,Shigella,and beta-hemolytic streptococci were lower than that of Camellia oleifera meal and the MBC values of extracts on Staphylococcus epidermidis,Staphylococcus aureus and beta hemolytic were lower than that of Camellia oleifera meal. 2 The detergent power of the related Traditional Chinese medicine prescription extracts was significantly higher than that of the Camellia oleifera meal extract,with the concentration of 0. 117 kg·L~(-1)crude drug being the most significantly different. Conclusion: The antibacterial role of the Traditional Chinese medicine prescription extracts including Camellia oleifera meal are better than that of Camellia oleifera meal working

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期刊信息
  • 《湖南农业大学学报:自然科学版》
  • 北大核心期刊(2011版)
  • 主管单位:湖南农业大学
  • 主办单位:湖南农业大学
  • 主编:官春云
  • 地址:长沙市芙蓉区农大路1号
  • 邮编:410128
  • 邮箱:zkb4618035@hunan.edu.cn
  • 电话:0731-84618035
  • 国际标准刊号:ISSN:1007-1032
  • 国内统一刊号:ISSN:43-1257/S
  • 邮发代号:42-157
  • 获奖情况:
  • 2006、2008、2010年连续三届全国高校精品科技期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,德国数学文摘,英国科学文摘数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:17328