中文摘要：

MyoD， Myf5，和 myogenin 是 myogenic 在 myogenesis 期间起重要作用的规章的因素。MyoD 和 Myf5 为 myogenic 决心被要求，这被认为，当 myogenin 为终端区别和系维护是重要的时。更好理解 myogenic 的函数在肌肉开发的规章的因素挣扎，在亚洲的一条重要的经济鱼，即时量的 RT-PCR 被用来在胚胎开发的早阶段描绘 MyoD， Myf5，和 myogenin 的表达式模式，并且在成年人的不同纸巾挣扎。结果显示出那， Myf5 是在早阶段期间要表示的第一基因挣扎开发，由 MyoD 和 myogenin 列在后面。在早阶段的 Myf5， yoD，和 myogenin 的表情有一个普通特征：逐渐地增加到山峰水平，然后逐渐地减少了到的表示一极其低级。在成年人挣扎，在肌肉的三基因的表示比的高得多在另外的纸巾，显示他们为肌肉生长和成年的鱼的维护是重要的。在胚胎的阶段期间， MyoD 的表示水平可能处于在肌肉房间区别和增长之间的平衡起一个重要作用。当 MyoD 表示是超过 30% 它的高水平时，肌肉房间进入区别阶段。关键词 Paralichthys olivaceus - myogenic 规章的因素 - myogenesis - 肌肉 - 量的 RT-PCR 由试验性的海洋的生物学的关键实验室支持了，中研院， Qingdao，中国；中国(863 节目)( 号码 2006AA10AA402 ) 的国家高技术研究和发展节目，中国(973 节目) 的国家基本研究节目(Nos. 2004CB117402， 2010CB126304 ) ，并且中国(号码 30871929 ) 的国家自然科学基础

英文摘要：

MyoD, Myf5, and myogenin are myogenic regulatory factors that play important roles during myogenesis. It is thought that MyoD and Myf5 are required for myogenic determination, while myogenin is important for terminal differentiation and lineage maintenance. To better understand the function of myogenic regulatory factors in muscle development of flounder, an important economic fish in Asia, real-time quantitative RT-PCR was used to characterize the expression patterns of MyoD, Myf5, and myogenin at early stages of embryo development, and in different tissues of the adult flounder. The results show that, MyJ5 is the first gene to be expressed during the early stages of flounder development, followed by MyoD and myogenin. The expressions ofMyf5, MyoD, and myogenin at the early stages have a common characteristic： expression gradually increased to a peak level, and then gradually decreased to an extremely low level. In the adult flounder, the expression of the three genes in muscle is much higher than that in other tissues, indicating that they are important for muscle growth and maintenance of grown fish. During embryonic stages, the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation. When the MyoD expression is over 30% of its highest level, the muscle cells enter the differentiation stage.