中文摘要：

目的研究WASP家族富含脯氨酸同源蛋白1（WAVE1）对K562细胞侵袭的影响及其机制。方法免疫荧光观察WAVE1与基质金属蛋白-2（MMP-2）在细胞中的分布。利用pcDNA3．1-WAVE1重组真核表达质粒转染K562细胞，将WAVE1基因特异性小片段干扰RNA（WAVE1 siRNA）转染K562细胞，Transwell法检测细胞侵袭能力；实时PCR和Western blot检测转染前后WAVE1及MMP-2的表达。结果①WAVE1与MMP-2主要表达于K562细胞的细胞膜上且两者有共定位。②与对照组K562细胞相比，转染pcDNA3．1-WAVE1 24h及48h后K562细胞MMP-2 mRNA表达分别增加295％和198％，蛋白表达水平分别增加80％和23％；转染特异性WAVE1 siRNA 24h及48h后K562细胞MMP-2 mRNA表达分别下降81％和28％，蛋白表达分别下调36％和53％。③与对照组K562细胞相比转染pcDNA3．1-WAVE1后细胞侵袭能力增强，而干扰WAVE1表达后K562细胞侵袭能力减弱。结论WAVE1与MMP-2在K562细胞可能具有协同作用；WAVE1参与了K562细胞的侵袭转移过程，其机制可能与调控MMP-2的表达相关。

英文摘要：

Objective To investigate role of WASP family verprolin homologous protein 1 （ WAVE1 ） in K.562 leukemia cell invasion and its mechanism. Methods Immunofluoresence methed was used to detect the distribution of WAVE1 and MMP-2 in the cells. K562 cells were transfected with pcDNA3.1-WAVE1 reconstructed plasmid or with specific siRNA to WAVE1 gene. The invasion ability of K562 cells was examined by Transwell assay. The expression level of WAVE1 and MMP-2 in K562 cells was assayed by real-time PCR and Western blot. Results ①WAVE1 and MMP-2 mainly expressed and co-localized in the cell membrane; ②24 h and 48 h after transfected with peDNA3.1-WAVE1, the MMP-2 mRNA level in K.562 cells increased by 295% and 198% while its protein increased by 80% and 23% respectively as compared with control K562 cells. At the same time point offer transfected with specific siRNA, the MMP-2 mRNA level decreased by 81% and 28% , and its protein decreased by 36% and 53% respectively as compared with control. ③The in- vasion ability of K562 cells was enhanced after transfected with pcDNA3.1-WAVE1 and depressed after transfected with the specific siRNA. Conclusion The co-localization of WAVE1 and MMP-2 in K562 cells suggests they coordinate in functions; WAVE1 may involve in the migration and invasion of K562 cells through regulating the expression level of MMP-2.