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Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet
  • ISSN号：1005-0302
  • 期刊名称：Journal of Materials Science and Technology
  • 时间：0
  • 页码：1119-1126
  • 分类：TQ651.2[化学工程—精细化工] O657.32[理学—分析化学;理学—化学]
  • 作者机构：[1]广西大学化学化工学院,广西南宁530004
  • 相关基金：广西自然科学基金项目（桂科50832002）.广西教育厅科研项目（200807MS149）
  • 相关项目：共固定化TNF-α / IFN-γ高抑制HeLa的分子机理
作者： Guan, Yanqing|Li, Zhibin|Wang, Xin|Ni, Xiaoli|Yang, Aini|Liu, Junming|
关键词： 肉桂, 总黄酮, 分离纯化, 大孔吸附树脂, cinnamom total flavonoids； separation and pufificatiom macroreticular resin
中文摘要：

采用大孔吸附树脂法分离纯化肉桂总黄酮，考察影响树脂静态和动态吸附与洗脱主要因素，确定肉桂总黄酮分离纯化优化工艺条件。结果表明：HPD．500大孔吸附树脂分离纯化效果较好，其对肉桂总黄酮静态吸附和解吸最佳工艺条件为吸附平衡时间6h、解吸平衡时间2h、样液质量浓度0．8mg／mL、样液pH6．0、洗脱剂乙醇体积分数80％；其对肉桂总黄酮动态吸附和洗脱最佳工艺条件为上样液质量浓度1．2mg／mL、上样液流速1．5mL／min、洗脱剂流速2．0mL／min、洗脱剂（80％乙醇）体积45mL时洗脱完全。在此条件下，分离纯化得到总黄酮含量为91．81％。

英文摘要：

The purification of total flavonoids from cinnamon was studied using macroreticular resin. Critical process variables that influence the static and dynamic adsorption and desorption of total flavonoids were selected and optimized. HPD-500 type macroreticular resin was found to be optimal for purifying total flavonoids from cinnamon. The optimal experimental conditions for static adsorption and desorption were determined as 6 h, 2 h, 0.8 mg/mL, 6.0 and 80% ethanol for adsorption time, desorption time, sample concentration, sample pH and desorption solvent, respectively. The optimal experimental conditions for dynamic adsorption and desorption were loading of 1.2 mg/mL sample solution at pH 6.0 with a flow rate of 1.5 mL/min and complete desorption with 45 mL of 80% ethanol at a flow rate of 2.0 mL/min. The content of total flavonoids in purified products under the optimized conditions was 91.81%.

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期刊信息
  • 《材料科学技术学报：英文版》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国金属学会
  • 主编：
  • 地址：中国沈阳文化路72号
  • 邮编：110016
  • 邮箱：
  • 电话：024-83978208
  • 国际标准刊号：ISSN：1005-0302
  • 国内统一刊号：ISSN：21-1315/TG
  • 邮发代号:
  • 获奖情况:
  • 国家“双百”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘（网络版）,荷兰文摘与引文数据库,美国工程索引,美国剑桥科学文摘,美国科学引文索引（扩展库）,日本日本科学技术振兴机构数据库,中国中国科技核心期刊
  • 被引量:474