利用硅胶和Sephadex LH-20等色谱分离手段从分布于秦岭的玉竹根茎中分离得到4个母核结构碳甲基化和氧甲基化的高异黄烷酮化合物,通过理化性质和光谱学方法及与文献值对照,鉴定化合物的结构分别为甲基麦冬黄烷酮B(Ⅰ)、4’,5,7-三羟基-6,8-二甲基高异黄烷酮(Ⅱ)、4’,5,7-三羟基-6甲基-8甲氧基高异黄烷酮(Ⅲ)和4’,5,7-三羟基-6-甲基高异黄烷酮(Ⅳ);其中化合物Ⅰ和化合物Ⅳ首次从黄精属植物中得到。
Column chromatography of silical gel and Sephadex LH-20 methods were used to isolate and purify the chemical constituents from the rhizome of Polygonatum odoratum. The four known rare C-methylated and O-methylated homoisoflavanones were isolated and characterized. The structure of the compound were elucidated by spectroscopic and the data reported in literature. The four methyl-homoisoflavanones were determined as 4' -methoxy-5,7-dihydroxy-6,8-dimethyl-homoisflavanone ( Ⅰ ), 4', 5,7-trihydroxy-6,8-dimethyl-homoisflavanone ( Ⅱ ), 4', 5,7-trihydroxy-6-methyl-8-methoxy-homoisflavanone ( Ⅲ) and 4' , 5,7-trihydroxy-6-methyl- homoisflavanone( Ⅳ ). Compound Ⅰ and Ⅳ were isolated from the Polygortatum genus for the first time.