测定了辽宁东港、广东大亚湾和广西东兴3个海域各15尾真鲷的线粒体控制区5’端660bp的核苷酸序列,发现91个可变位点,64个简约信息位点。在45尾样品中共检测到43个单倍型,3个群体的平均单倍型多样性和核苷酸多样性分别为(0.99-1)和(0.02522-0.02579),表现出较高的单倍型多样性和核苷酸多样性。在真鲷个体NJ树上出现3个谱系,各谱系中不同地理来源的个体比例相当,不呈现明显的地理聚群,推测各谱系大约形成于晚更新世的末期。Tajima’s D呈负值但不显著(P>0.08),表明真鲷历史上没有发生快速扩张,种群大小稳定。群体间遗传分化指数Fst都为负值(-0.0001-0.0188)但不显著(P〉0.1);AMOVA分析显示遗传变异主要集中在群体内的个体间(100.49%),而群体间的遗传变异很小(-0.49%)表明我国近海真鲷群体有可能是同一种群。
To analyze genetic variation of red seabream population in coastal waters of China, 660 bp DNA sequences at 5'- terminal mtDNA control region of 45 individuals sampled from 3 localities including Donggang in Liaoling province, Dayawang in Guangdong province, and Dongxin in Guangxi province were determined. Totally 91 variable sites and 64 parsimony informative sites defined 43 haplotypes among 45 individuals. The average of haplotype diversity and nucleotide diversity among 3 geographic group were 0.99-1 and 0.0252-4).0257 respectively, indicating both high haplotype and nucleotide diversity. In the Neighbor- Joining tree 3 lineages were found without obvious geographic groups, as the ratio of individuals from different locality within each lineages were similar. The deduced speeiation time of 3 lineages were at the end of late Pleistocene. Tajima's D were negative but not significant(P〉0.08), indicating a stable population size without rapid expanding. Pair-wise genetic differentiation index Fst were negative (-0.00148-0.01770) but not significant (P〉0.1);genetie variation within and among populations were 100.49% and - 0.49% respectively in AMOVA analysis;it were suggested that red seabream populations in Donggang, Dayawan and Dongxing along coastal waters of China might be treated as one population.