中文摘要：

melittin 的抗菌剂效果的光谱被与连续冲淡使用一个柱体板方法在 19pathogens 上调查。细菌抑制的效率和可能的机械学的效果经由生长曲线被监视。抑制的机制被 SDS 页，流动式细胞测量术和电子显微镜学进一步分析。Melittin 有一个宽抑制系列并且有效地杀死了病原体，并且细菌抑制的行动被象酸碱值和温度那样的因素影响。我们阐明了三禁止的机制：与引起房间爆炸和细胞质版本的房间膜综合的 melittin，禁止了蛋白质的合成并且引起了细胞质压缩，并且在阶段推迟了病原体我(或阶段 G1 ) 以便他们不能完成房间周期。这些结果建议那 melittin 能用作 broad-spectrumbiological 杀虫剂与快行动、高效率。

英文摘要：

The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylinder-plate method with serial dilutions. BacteriostaUc efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. MelitUn had a wide inhibition spectrum and killed pathogens effectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci- dated three inhibitory mechanisms： melitUn integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase Ⅰ（or phase G1） so that they could not complete the cell cycle. These resuits suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.