中文摘要：

目的探讨氨溴索对铜绿假单胞菌生物膜群体感应（Qs）系统的影响。方法平板法培养成熟的铜绿假单胞菌PA01野生型菌株和Qs系统缺陷株△lasR△rhlR基因缺陷型、△lasI△rhlI基因缺陷型菌株生物膜，分别予生理盐水和氨溴索1．875g·L^-1、3．75g·L^-1作用8h。通过荧光探针SYT09／PI标记，激光共聚焦显微镜（CLSM）观察不同浓度氨溴索作用前后不同菌株生物膜的结构变化，利用生物膜图象结构分析软件（ISA）对氨溴索作用前后的生物膜结构参数进行定量分析。结果PA01野生型菌株可见大片状生物膜，细菌密集。2个基因缺陷型菌株的生物膜菌落较稀疏，氨溴索作用后不同菌株生物膜厚度、平均扩散距离和结构熵均显著减少，区域孔率增加，有剂量相关性（P〈0．05），PA01野生型菌株生物膜的变化幅度比QS系统缺陷株更大（P〈0．05）。结论氨溴索破坏铜绿假单胞菌生物膜的结构，具有拮抗QS系统的特性，高浓度氨溴索（3．75g·L^-1）效果更显著。

英文摘要：

AIM To investigate the effect of ambroxol on biofilm quorum-sensing system of Pseudomonas aeruginosa. METHODS In vitro biofilm models of Pseudomonas aeruginosa PAO1 wild-type, △lasR △rhlRand △lasI △rhlI isolate strain were established. The effects of normal saline and ambroxol 1.875 g·L^-1 and 3.75 g·L^-1 on biofilm were monitored by confocal laser scanning microscopy （CLSM） respectively, based on nucleic acid SYTO9/PI stained. Quantitative parameters describing biofilm spatial structure were acquired after the image information and calculated by Image Structure Analyzer （ISA） software. RESULTS The PAO1 wild-type strain produced a significantly thicker biofilm compared to biofilms produced by the △rhlR and △lasl △rhll isolates which appeared more diffuse, respectively. Ambroxol treatment reduced biofilm thickness, increased areal porosity, decreased the average diffusion distance and the textual entropy of wild-type and the two doublemutant strains （P 〈 0.05）. However, compared to the double mutant strains, the changes observed for the wildtype strain were more clearly defined （P 〈 0.05）. CONCLUSION Ambroxol destroys the structure of biofilm and exhibits significant antagonistic quorum-sensing property, and its high concentration （3.75 g·L^-1） has more significant effect.