中文摘要：

目的探讨低频超声联合万古霉素对表皮葡萄球菌假epidermidis）RP62A生物被膜（biofilm，BF）的影响。方法建立兔表皮葡萄球菌导管感染模型，设空白对照组，单独低频超声组，单独万古霉素组，低频超声联合万古霉素组。涂板菌落形成单位（colony．formingunits，CFU）计数法计算导管细菌数；扫描电镜（scanningelectronmicroscope，SEM）观察BF结构的改变；皮肤、心脏、肝脏及肾脏病理组织切片检查组织的炎症反应。结果单独低频超声对BF内细菌无杀灭作用，单独万古霉素能比较有效地杀灭BF内细菌，较空白对照组CFU减小，P〈0．01；低频超声联合万古霉素组效果更加明显。SEM显示低频超声对BF结构无影响；与各单独作用组相比，低频超声联合万古霉素能明显地破坏BF，使其结构变得更加疏松。皮肤病理组织切片显示低频超声联合万古霉素组，皮下隧道炎症明显减轻。结论低频超声联合万古霉素能更显著地清除导管表皮葡萄球菌BF。

英文摘要：

Objective To investigate the effect of low-frequency ultrasound combined with vancomycin on S. epidermidis RP62A strain＇s biofilm（BF）. Methods Rabbit model of S. epidermidis catheter infection was established, including blank control group, low-frequency ultrasound alone group, vancomycin alone group and low- frequency ultrasound combined with vancomycin group. Coated plate colony forming units （CFU） counting method was used to calculate the number of bacteria on catheter; Changs in the structures of BF were observed by scanning electron microscope（SEM）; pathology biopsy of skin, heart, liver and kidney to test tissue inflammation. Results Low-frequency ultrasound alone had no effect in killing BF bacteria. Vancomycin alone could eradicate BF bacteria more effectively. Compared with the control group, CFU decreased, P〈0.01; The effect of low-frequency ultrasound combined with vancomycin group was more obvious. SEM showed that the low-frequency ultrasound han no effect on the structure of BF. Compared with the alone groups, low-frequency ultrasound combined with vancomycin might significantly undermine BF, and made its structure became more loose. Conclusion Low-frequency ultrasound combined with vancomycin can more efficiently eradicate Staphylococcus epidermis BF on catheter.