中文摘要：

目的对比不同来源间充质干细胞（MSC）形态的差异,观察骨折大鼠血清对不同来源MSC的趋化作用。方法将36只SD大鼠完全随机地分为骨折组（24只,其中12只培养外周血MSC,12只分离血清）和对照组（12只）,从两组大鼠骨髓和外周血中分离培养MSC,比较生长速度和细胞形态。观察两组血清对MSC的趋化作用及不同来源MSC的迁移能力。结果两组大鼠骨髓均可培养出MSC。骨折组12只大鼠外周血中有9只培养出MSC,其阳性率低于骨髓（75%对100%）,各组MSC形态无明显差异。骨折组大鼠血清处理的MSC迁移数明显多于对照组大鼠血清处理者（P〈0.01）。对照组大鼠骨髓MSC被骨折大鼠血清趋化时迁移的细胞数明显少于骨折组骨髓MSC和外周血MSC（P〈0.01）。骨折组大鼠骨髓MSC与外周血MSC的迁移能力也存在明显差异（P〈0.01）。结论骨折大鼠血清对MSC有较强的趋化作用,骨折大鼠来源的MSC比对照组来源的MSC具有更强的迁移能力,其中骨折大鼠外周血来源的MSC迁移能力最强。

英文摘要：

Objective To compare the morphology of mesenchymal stem cells（MSCs）from bone marrow with from peripheral blood and investigate the chemotactic effects of serum from rats with fracture on MSCs derived from different sources. Methods Thirty-six Sprague-Dawley rats were randomly divided into 2 groups：fracture group（n=24,of with MSCs from peripheral blood were cultured from 12 rats,serum was separated from others）,control group（n=12）.Bone marrow and peripheral blood of rats in both groups were collected to isolate and culture MSCs.Growth speed and cell morphology of MSCs were observed.Effects of both serums on chemotaxis of MSCs derived from different sources and their migration ability were tested with a Transwell system.Results MSCs were successfully obtained from bone marrow of all rats in both groups,but only from peripheral blood of 9 rats with fracture（100%vs 75%）.There was no obvious difference in the cell morphology between the both groups.The number of MSCs migrating to the lower surface of Transwell plate after treatment with serum from rats with fracture was significantly higher than that after the treatment with control rat serum（P0.01）.The migration rate of MSCs which were derived from bone marrow in control group was obviously lower than those derived from bone marrow and peripheral blood from rats with fracture（P0.01）.Difference of the migration ability existed between MSCs derived from bone marrow and peripheral blood in fracture group（P0.01）.Conclusions Serum from rats with fracture has a stronger chemotactic effect on MSCs. Moreover,the migration ability of MSCs derived from rats with fracture is stronger than that of MSCs derived from control rats, while the migration ability of MSCs from peripheral blood of rats with fracture is the strongest.