中文摘要：

本研究旨在探讨大鼠短暂性脑缺血损伤后不同时间点脑皮层中神经突起生长因子（neuritin）表达的变化规律,为研究neuritin在神经修复中的作用奠定基础数据。采用夹闭双侧颈总动脉的方法制作TGI大鼠模型,按伤后不同时间分为3、7、14、21d组,用免疫印迹及定量PCR的方法检测大鼠脑皮层neuritin蛋白质及mRNA的表达,并分析其表达变化特点。免疫印迹结果显示：3d、21d实验组皮层neuritin的表达与对照组相比无明显变化;7d和14d实验组Neuritin蛋白表达量,显著高于3、21d和对照组。Neuritin mRNA定量PCR结果表明：与对照组相比,各实验组无明显变化。基于Neuritin mRNA和蛋白质的表达特点,推测可能通过某种转录后调控机制调控Neuritin蛋白表达的变化。TGI后,机体启动神经修复机制,Neuritin蛋白表达迅速升高,并维持两周较高水平的表达。当修复完成后Neuritin又恢复到正常水平。这种变化规律提示neuritin在神经损伤中可能具有重要的修复作用。

英文摘要：

To detect time-dependent change of neuritin expression after transient global ischemia （TGI） injury at different time points and provide fundamental data for the research into the function of neuritin in nerve reconstruction. The model of TGI was established by occluding the double common carotid arteries （CCAs） for 15 min in SD rats. Western Blot and qPCR were used to analyze the characteristics of neuritin expression at different time points （3,7,14,21d） in rat cortex. The results indicated that the level of neuritin protein expression sharply increased and reached the peak at day 7. Compared with protein, the level of neuritin mRNA did not change between test groups and its control. Based on the expression of neuritin protein and mRNA,we inferred that the change of protein might be controlled by post-transcriptional regulation. Self-repair systems would start nerve reconstruction after TGI, the expression of endogenous Neuritin protein increased quickly,then maintained in a higher level for two weeks and returned to the normal level at day 21 in SD rat cortex. These suggested that neuritin probably might play an important role in the recovery of cortex.