中文摘要：

目的研究较长时间提取和浓缩对琼玉膏品质的影响,探讨琼玉膏主成分在提取和浓缩过程中的降解、转化机制。方法采用HPLC-MS方法同时测定琼玉膏中主要活性成分5-羟甲基糠醛、梓醇、密力特苷、毛蕊花糖苷、人参皂苷Re、人参皂苷Rb1、人参皂苷20（S）-Rg3、人参皂苷Rg1、人参皂苷Ro和茯苓酸,比较不同提取和浓缩时间重复制备样品中10种成分质量分数标准差（SD）的累加值,考察提取和浓缩时间对琼玉膏品质的影响。结果随着提取和浓缩时间的延长,10种活性成分的总量和部分成分的相对质量分数显著改变,同时重复制备样品质量分数误差累加值随提取和浓缩时间延长呈下降趋势。结论提取和浓缩时间可显著影响琼玉膏活性成分转化程度,从而影响其品质的一致性。

英文摘要：

Objective To explore the influence of extraction and concentration with long duration on the quality consistency of Qiongyu Paste（QYP）, and to analyse the degradation and transformation mechanisms of each component involved in the quality change of QYP. Methods QYP was a paste formula derived from Rehmanniae Radix, Poria, and Ginseng Radix et Rhizoma in a weight ratio of 7∶2∶1, the contents of 10 major bioactive components [5-hydroxymethyl furfural（5-HMF）, catalpol, melittoside, acetoside, ginsenoside Re, ginsenoside Rb1, ginsenoside 20（S）-Rg3, ginsenoside Rg1, ginsenoside Ro, and pachymic acid] were simultaneously determined by the previously established HPLC-MS method. The standard deviation（SD） accumulation values of the contents of 10 bioactive components in repeatedly prepared samples in different durations were compared. Results Total contents of the 10 components and relative contents of some individual components in QYP changed significantly with different extraction and concentration duration. At the same time, the SD values of the contents of bioactive components in repeatedly prepared samples decreased with extending the extraction and concentration duration. Conclusion Extraction and concentration could improve the quality consistency of QYP.