对披针叶黄华(Thermopsis lanceolata)中分离的编号为SP01、RM02的2株蠕形分生孢子内生真菌进行种属分类鉴定。观察真菌的形态,提取真菌DNA,扩增并测定其ITS、GAPDH序列,构建系统发育树,结合形态特征和遗传进化分析结果,并对其进行种属分类。结果表明,SP01菌株的形态特征与凸脐孢属(Exserohilum)中的E.rostratum最为相似,与E.rostratum的遗传进化关系最为密切;RM02菌株的形态特征与弯孢属(Curvularia)中C.spicifera、C.australiensis最为相似,与C.spicifera的遗传进化关系最为密切。确定披针叶黄华中分离的SP01、RM02分别为E.rostratum和C.spicifera。
This study was conducted to confirm the species of two helminthosporioid fungal endophytes, SP01 and RM02,isolated from wild Thermopsis lanceolata. The two isolates were observed under the light microscope respectively. The DNA of the fungi were extracted for amplifying the fungal ITS and GAPDH sequences that were sequenced subsequently. Homology comparison was conducted and NJ phylogenetic trees were established based on the sequences of the fungi. Two fungal endophytes were classified according to the fungal morphology and phylogenetic analysis. The results indicated that SP01 was similar to Exserohilum rostratum. Homology and phylogenetic analyses also showed SP01 to be more closely related to E. rostratum. RM02 was more similar to some fungi of Curvularia genus, such as C. spicifera and C. australiensis. Homology and phylogenetic analyses also showed the isolate to be most closely related to C. spicifera. In addition to above results, the two isolates, SP01 and RM02, were classified as E. rostratum and C. spicifera respectively.