中文摘要：

研究不同浓度邻苯二甲酸二丁酯（DBP）对三疣梭子蟹相关酶活的影响及组织损伤。设置了三个处理浓度（0.03 mg/L、0.2 mg/L和0.4 mg/L）分别在第0、5、10、15、20 d进行取样。结果表明,随着DBP浓度的增大,三疣梭子蟹死亡率升高。在0.03 mg/L浓度组胁迫下,血清超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和碱性磷酸酶（AKP）活性呈现升高-下降交替波动,过氧化物酶（POD）活性在第10 d低于对照组（P〈0.05）,其余时间与对照组持平,酸性磷酸酶（ACP）活性在第5 d较对照组显著升高（P〈0.05）,此后一直保持对照组水平;在0.2 mg/L浓度组胁迫下,血清SOD活性呈现先升高后下降的趋势,POD活性变化不明显,CAT活性先下降后升高,此后恢复到对照组水平,ACP活性在第15 d出现最高值,其余时间与对照组持平,AKP活性呈现先升高后恢复的波动趋势;0.4 mg/L浓度组SOD、CAT和AKP活性呈现先升高后下降的趋势,POD活性和ACP活性在第20 d较对照组低（P〈0.05）,其余时间与对照组持平。组织切片观察可见,DBP胁迫损伤了三疣梭子蟹肝胰腺和鳃组织,DBP浓度越大,胁迫时间越长,损伤越明显。

英文摘要：

Effects of dibutyl phthalate（DBP） with different concentrations on related enzyme activities and tissue of Portunus trituberculatus were researched. Crabs were sampled at 0,5,10,15 and 20 d after stressing by three different concentrations. The results showed that the mortality increased with the increasing of DBP concentration. In 0.03 mg/L DBP treatment,SOD CAT and AKP activities showed an alternate fluctuation of increase and decrease. POD activity was lower than the control group at 10 d（P0.05） and was equivalent to the control group in other times. However,ACP activity was significantly higher than the control group at 5 d（P0.05）and maintained the level of the control group until the end of the experiment. Nevertheless,SOD activity was first increased and then showed a downward trend while the concentration was 0.2 mg/L. Although POD activity almost remained at the same level with the control group,CAT activity first decreased and then increased,and finally restored to same level of control group. ACP activity increased to the highest value in 15 d and was equal to the control group in other times. However,AKP activity first raised then recovered,showing a fluctuating trend. When the concentration was 0.4 mg/L,SOD,CAT and AKP activities showed a trend of first increasing and then decreasing. POD and ACP activities were lower than those in the control group in 20 d（P0.05）,but were equivalent to the control group in other times. Furthermore,histological examination indicated that the hepatopancreas and gill of P.trituberculatus were damaged by DBP. In addition,the higher the concentration was and longer the stress time was,the more obvious the damage was.