中文摘要：

目的：应用功能分类基因芯片研究脑动-静脉畸形（BAVM）不同结构部位的血管生成相关基因表达.方法：6例BAVM,分别在“供血动脉”、“引流静脉”、“畸形团血管”和“周边脑组织”4个不同部位取材,进行血管新生芯片杂交.以“周边脑组织”为自身对照,计算“畸形团血管”、“供血动脉”和“引流静脉”与之的比值.随意选取8个基因进行逆转录-聚合酶链反应（RT-PCR）验证.结果：经过分析和比较,共筛选出44个明显异常的基因,包括了生长因子和受体相关基因18个（含4个下调基因）,黏附分子相关基因6个,基质蛋白相关基因6个,转录因子基因5个,细胞因子和趋化因子基因9个（含1个下调基因）.RT-PCR结果与之基本一致.结论：为BAVM的机制研究开辟了许多新的切入点。

英文摘要：

ABSTRACT Aim： A typical human brain arteriovenous malformation （BAVM） is composed of arterial, nidal and venous parts. We examined angiogenesis gene expression patterns at these different parts in BAVM by the focused DNA microarray technique. Methods： We analyzed arterial, nidal, venous and adjacent brain tissue from six resected BAVMs by quantifying mRNA expression levels on Oligo GEArray Human Angiogenesis Microarray OHS-024 （SuperArray, Inc., Frederick, MD）. Adjacent brain tissue is as self control. Results. By comparison, 44 of 113 angiogenesis related key genes are found differential expression. Our focus is mainly on nidus, but the results also showed distinct expression patterns of the other parts in BAVM. Conclusion： Focused DNA microarray analysis is a promising way to find distinct gene expression patterns of different parts of BAVM by self-contrast.