中文摘要：

目的 探讨17-β雌二醇治疗脊髓损伤的分子机制，为临床应用雌激素治疗脊髓损伤提供理论依据。 方法 成年雄性SD大鼠180只，采用改良的Allen法构建大鼠急性脊髓挫伤模型后经17-β雌二醇治疗，采用化学比色法测定脊髓组织中丙二醛（MDA）和内源性巯醇抗氧化物（酶）：还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）含量；免疫组织化学分析凋亡相关因子Caspase-3、Bcl-2和Bax蛋白的表达变化。 结果 17-β雌二醇治疗组与脊髓损伤组比较，BBB评分明显增加；在多个时间点MDA含量明显降低；巯醇抗氧化物（酶）GSH、SOD和GSH-Px含量显著增加；Caspase-3和Bax表达的阳性细胞数明显减少；Bcl-2表达的阳性细胞数明显增加（P〈0.05）。 结论 大剂量的17-β雌二醇治疗可能通过调控内源性巯醇抗氧化物（酶），提高肢体运动功能，抑制细胞凋亡，从而减轻脊髓损伤程度。

英文摘要：

Objective To examine the protective effects of 17-β estradiol on the experimental model of spinal cord injury （SCI） rats. Methods One hundred and eighty male Sprague Dawley （SD） rats, after Allen’s model, SD rats were divided into three groups： the sham group, the acute spinal cord injury （control groups） and the acute spinal cord injury supplying with 17-β estradiol treatment group. SCI was made by Allen’s weight dropping, impacting on the posteriors of spinal cord T10.The content of malonyldialdehyed （MDA）, glutathione （GSH）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were determined by chromatometry. The expressions of Caspase-3 and Bcl-2 family in the injured spinal cord were detected by immunohistochemical staining. Results The BBB scores at each time point in 17-β estradiol treatment group were significantly higher than that in SCI group （P〈0.05）. The contents of GSH, SOD, GSH-Px and the expression of Bcl-2 protein at the majority of time point in 17-β estradiol treatment group were significantly higher than that in SCI group（P〈0.05）, however, the MDA, Caspase-3 and Bax were markedly decreased （P〈0.05）. Conclusions This study suggests that 17-β estradiol administration might prevent the cells from SCI-induced apoptosis by triggering to reduce the oxidative stress.