中文摘要：

探讨17—β雌二醇（17β-Estradiol，17β-E2）对高同型半胱氨酸（High Homocystine，HHcy）诱导的破骨前体细胞株Raw264．7炎性因子释放的抑制作用．用同型半胱氨酸（Homocystine，Hcy）刺激Raw264．7细胞构建炎症模型，采用四甲基偶氮唑蓝比色法（MIT）检测17β—E2对Raw264．7细胞的活力影响，免疫荧光双标和RT—PCR方法检测不同浓度17β-E2（1，10nmol／L和1μmol／L）对环氧合酶-2（COX-2）和细胞炎性蛋白酶诱导型一氧化氮合酶（iNOS）、细胞炎性因子肿瘤坏死因子-α（TNF-α）和白介素-1β（IL-1β）、炎性信号蛋白核因子-κB（NF—κB）蛋白与mRNA的表达变化．结果发现：不同浓度的17β-E2在翻译水平和转录水平上明显抑制了Hcy诱导的细胞炎性蛋白酶COX-2和iNOS，细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调，并且COX-2和IL-1β蛋白和mRNA的表达呈剂量依赖性．上述结果表明17β-E2可通过调控Hcy诱导的破骨前体细胞株Raw264．7细胞炎性因子释放从而抑制破骨激活，发挥抗骨质疏松的作用．

英文摘要：

To investigate suppressing effects of 17β - Estradiol （ 17β - E2 ） in the activation of Raw 264.7 cells,the cells were treated with 17β -E2 prior to Homocystine （Hcy） exposure,then the effects on the mRNA and protein levels of pro - inflammatory enzymes, cyclooxygenase - 2 （ COX - 2） and inducible nitric oxide synthase （ iNOS）, and pro - inflammatory cytokines, tumor necrosis factor - α（ TNF - α）, and interleukin - 1β （ IL - 1β ）, inflammatory signaling proteins nuclear factor - κB （ NF - κB） were analysed by reverse transcription - polymerase chain reaction （RT- PCR） and double -immunofluorescence labeling assay,and the effects of 17β - E2 on viability of Raw 264.7 cells were measured by MTT assay. The results showed that 17β - E2 had an effect on COX - 2, iNOS, TNF - α, IL - 1β, NF - κB protein and mRNA expression levels. Arising from the above ,we think 17β -E2 can restrain osteoclasts activity by means of regulation Hcy- induced pro -inflammatory mediators in Raw 264.7 cells, and exert its anti -osteoporosis actions.