中文摘要：

以辣椒（Capsicum annuum L．）双单倍体HDA149为试验材料，接种南方根结线虫（Meloidogyne incognha）12、24、36h的根尖材料作为测验方（Tester），相应的未接种根尖材料作为驱动方（Driver），构建一个南方根结线虫诱导Me3基因表达早期的抑制消减杂交cDNA文库，通过高密度点阵膜杂交差异筛选，获得了309条表达序列标签（EST）。通过测序，除去重复序列共得到259条EST序列，在Gen—Bank上进行BLAST分析，30个EST片段未发现同源性基因，229个为具有同源性的已知基因，其中71个为功能未知的基因。在显微观察和抗性相关EST表达谱分析的基础上，推测在Me5基因介导的不亲和互作中，编码NBS—LRR结构的抗病基因参与了寄主对根结线虫的识别，激发了过敏性坏死反应的信号基因，同时多种抗性相关的转录因子基因表达，使以代谢为基础的防御反应和保护机制发挥抗线虫作用。

英文摘要：

To investigate the early up-regulated expression gene profile in pepper line HAD149 induced by root-knot nematode （RKN） Meloidogyne incognita, a forward subtracted cDNA library of pepper seedling root tips was constructed using suppression subtractive hybridization （ SSH ）. The cDNA of pepper seedling root tips inoculated with J2 RKN on 12, 24 and 36 h were used as tester, and that from untreated root tips as driver. Totally 309 SSH cDNA fragments were selected with DIG Nonradioactive Nucleic Acid Labeling and Detection System. Through the DNA sequencing, we got 259 ESTs. With the Blast, 30 EST fragments didn＇t find homologous gene, 229 had homologous genes, and among of them, 71 fragments were function unknown. Base on the observing with microscope and the ESTs function analyzing, we putative that disease resistant genes encode nucleotide binding/leucine-rich repeat （ NBS - LRR） have a key rule to recognizes root knot nematode, result in the signal transduction-related genes expressed, activate a suitable defensive response and protection mechanism, it often including hypersensitive response （HR） and Systemic Acquired Resistance （SAR）. In addition, all of these responses are base on the first and secondary metabolites.