中文摘要：

瞄准：在老鼠肝在 mitochondrial 能代谢上探索比重低於脑脊髓液的组织缺氧的效果。方法：成年男 Wistar 老鼠暴露于高模仿 5,000 m 的一个比重低於脑脊髓液的房间为 23 h 的高度每天为 0 (H0 ) ， 1 (H1 ) ， 5 (H5 ) ， 15 (H15 ) 并且 30 d (H30 ) 分别地。老鼠被斩首杀头牺牲，肝被移开。肝线粒体被差示离心节目孤立。在织物和线粒体的腺嘌核苷酸水池(ATP，自动数据处理，和安培) 的尺寸被高效液相色谱法(HPLC ) 分开并且测量。腺嘌核苷酸 transporter (蚂蚁) 活动被同位素的技术决定。蚂蚁全部的蛋白质水平被西方的污点决定。结果：与 H0 组相比， intra-mitochondrial ATP 内容在所有组织缺氧组减少了。然而，当时， H5 组到达了最低的点(70.6%)(P【0.01 ) 与控制组相比。Intra-mitochondrial 自动数据处理和安培水平在所有组织缺氧组显示出类似的变化并且在 H0 组是比那显著地低的。另外， extra-mitochondrial ATP 和自动数据处理内容在所有组织缺氧组显著地减少了。而且，在组 H5， H15 和 H30 的 extra-mitochondrial 安培在 H0 组是比那显著地低的，而 H1 组不与控制状况相比标记变化。当时，在组织缺氧组的蚂蚁的活动显著地减少了，它在 H5 组(55.7%)(P【0.01 ) 是最低的与 H0 相比组。在 H30 组的蚂蚁活动比在 H15 组高，但是仍然在 H0 组比那降低。在 H5 的蚂蚁蛋白质水平， H15， H30 组，与 H0 相比，组显著地减少了，在 H5，组是它最低，在 H0 是那中的 27.1% 个组织(P【0.01 ) 。在 H30 组的蚂蚁蛋白质水平比在 H15 组高，但是仍然比在 H0 组降低。结论：比重低於脑脊髓液的组织缺氧减少 mitochondrial ATP 在老鼠肝满足，当 mitochondrial ATP 水平在长期的组织缺氧暴露期间恢复时。extra-mitochondrial ATP 的底层可能在组织缺氧暴露期间与蚂蚁活动的减少有关。

英文摘要：

AIM： To explore the effect of hypobaric hypoxia on mitochondrial energy metabolism in rat liver. METHODS： Adult male Wistar rats were exposed to a hypobadc chamber simulating 5000 m high altitude for 23 h every day for 0 （H0）, 1 （H1）, 5 （H5）, 15 （H15） and 30 d （H30） respectively. Rats were sacrificed by decapitation and liver was removed. Liver mitochondria were isolated by differential centrifugation program. The size of adenine nucleotide pool （ATP ADP, and AMP） in tissue and mitochondria was separated and measured by high performance liquid chromatography （HPLC）. The adenine nucleotide transporter （ANT） activity was determined by isotopic technique. The ANT total protein level was determined by Western blot. RESULTS： Compared with H0 group, intra-mitochondrial ATP content decreased in all hypoxia groups. However, the H5 group reached the lowest point （70.6%） （P〈 0.01） when compared to the control group. Intra-mitochondrial ADP and AMP level showed similar change in all hypoxia groups and were significantly lower than that in H0 group, In addition, extra-mitochondrial ATP and ADP content decreased significantly in all hypoxia groups. Furthermore, extra-mitochondrial AMP in groups H5, H15 and H30 was significantly lower than that in H0 group, whereas HI group had no marked change compared to the control situation. The activity of ANT in hypoxia groups decreased significantly, which was the lowest in H5 group （55.7%） （P〈0.01） when compared to H0 group. ANT activity in H30 group was higher than in H15 group, but still lower than that in H0 group. ANT protein level in H5, H15, H30 groups, compared with H0 group decreased significantly, which in H5 group was the lowest, being 27.1% of that in H0 group （P〈0.01）. ANT protein level in H30 group was higher than in H15 group, but still lower than in H0 group. CONCLUSION： Hypobaric hypoxia decreases the mitochondrial ATP content in rat liver, while mitochondrial ATP level recovers during long-term hypoxia e