中文摘要：

研究克隆了玉米大斑病菌（Setosphearia turcica）G蛋白β亚基编码基因Stgb-1。Stgb-1基因全长1296bp,由5个外显子和4个内含子组成;开放阅读框（ORF）为1056bp,编码351个氨基酸,蛋白质计算分子量为39.081kD。其推导氨基酸序列与Cochliobolus heterostrophus、Cryphonectria parasitica和Aspergillus fumigatus的G蛋白β亚基编码基因均具有较高的同源性,分别为100%、80%和81%。将该序列提交GenBank登录号为No.EF407555。利用RACE（rapid amplification of cDNA ends）和Genomic walking技术获得了Stgb-1基因3′末端cDNA和3′端DNA侧翼序列,BlastN结果表明其cDNA3′-UTR为136bp,poly（A）由9个A构成。构建了pET28a-Stgb-1原核表达载体,SDS-PAGE检测和Western blot鉴定结果表明,目的蛋白在大肠杆菌（Escherichia coli）BL21菌株中诱导表达。

英文摘要：

Stgb-1 gene from the phytopathogenic fungus of Setosphearia turcica, which encoded a protein of 351 amino acid residues, was 1 296 bp and interrupted by four introns. It shared a high degree of amino acid homology with Gβ protein from Cochliobolus heterostrophus, Cryphonectn＇a parasitica and Aspergillus fumigatus, which was 100%, 80% and 81%, respectively. The sequence has been deposited in the GenBank/EBI Database（accession No. EF407555）. Results of RACE （rapid amplification of eDNA ends） and Genomic walking showed a 136 bp 3′UTR in its 3′ end eDNA with a poly （A） tail containing nine adenylates. The pET vector system was used to express Stgb-1 in vitro. Results of SDS-PAGE and Western blot showed that the recombinant protein with the calculated molecular mass of 40 kD was expressed in Escherichia coll.