中文摘要：

目的研究疏肝清热健脾法对广泛性焦虑（GAD）大鼠脑内Papez环路区域的B细胞白血病/淋巴瘤-2（bcl-2）、B细胞白血病/淋巴瘤-2相关蛋白（bax）2种蛋白表达的影响。方法将40只雄性Wistar大鼠随机分为正常组、模型组、中药治疗组和西药对照组4组,每组10只大鼠。模型组、治疗组、对照组采用不确定空瓶饮水刺激方法制备广泛性焦虑大鼠模型。治疗组和对照组分别以丹栀逍遥散（10.5 g/kg）和盐酸丁螺环酮（2 mg/kg）水溶液灌胃7 d,每天1次。模型组蒸馏水灌胃7 d。取脑组织,western blot法检测大鼠脑内海马和乳头体组织bax和bcl-2的含量。结果与正常组比较,模型组海马和乳头体组织的bax含量显著增加,差异有统计学意义（P〈0.05或P〈0.01）,bcl-2的含量有较明显的下降,差异有统计学意义（P〈0.01）;与模型组相比,治疗组海马及乳头体组织的bax表达均有降低,差异有统计学意义（P〈0.05或P〈0.01）,bcl-2的表达均有增加,差异有统计学意义（P〈0.01）。结论以丹栀逍遥散为代表的疏肝清热健脾法能够降低GAD大鼠脑内海马和乳头体组织bax的水平,升高bcl-2的水平,降低bax/bcl-2值,干预和抑制Papez环路区域的细胞凋亡,从而改善焦虑症状。

英文摘要：

Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez＇s circuit in rat with generalized Anxiety Disease（ GAD）. Methods A total of 40 male Wistar rats were divided into normal group,model group,treatment group,and control group,with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragastrically given Danzhi Xiaoyao San（ Cortex Moutan and Fructus Gardeniae Free Wanderer Powder） and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given distilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group（ P〈0. 05 or P〈0. 01） and the expression of bcl-2 decreased markedly（ P〈0. 01）. Compared with model group,the level of bax in hippocampus and mamillary body of treatment group and control group was reduced（ P〈0. 05 or P〈0. 01） and the level of bcl-2 increased significantly（ P〈0. 05 or P〈0. 01）. Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain,elevate the level of bcl-2 and reduce bax / bcl-2,prevent and inhibit cell apoptosis in Papez＇s circuit and then ameliorate anxiety symptoms.