中文摘要：

目的：探讨苦参碱对高表达三磷酸腺苷（ATP）结合转运蛋白G超家族成员2（ABCG2）人耐药鼻咽癌细胞CNE2／DDP（简写作ABCG_2H^High CNE2／DDP）NKG2D配体表达的诱导作用及其对NK细胞杀伤敏感性的机制。方法：利用免疫磁珠技术分离ABCG_2^High CNE2／DDP细胞及NK细胞，流式细胞技术检测分离后细胞纯度及经苦参碱处理前后靶细胞NKG2D配体表达率，LDH释放测定法检测经苦参碱处理前后ABCG_2^High CNE2／DDP细胞对NK细胞的杀伤敏感性。结果：ABCG_2^High CNE2／DDP细胞分离后ABCG2表达率为（91．40±2．32）％，分选后NK细胞CD3^-CD16^＋CD56^＋细胞的纯度达90％以上，经苦参碱处理之后靶细胞MICA、MICB、ULBP1、ULBP2、ULBP3表达率，由药物处理之前的（2．92±0．33）％、（4．27±0．33）％、（5．80±0．62）％、（11．10±3．15）％、（7．75±1．14）％分别上升到（11．30±0．89）％、（14．29±2．61）％、（12．56±1．06）％、（43．24±4．43）％、（12．77±1．06）％。在效靶比为10：1、20：1时，NK细胞对苦参碱处理前后ABCG^2^High CNE2／DDP细胞的杀伤率分别为（15.32±1．34）％、（27．26±6．81）％及（28．53±1．37）％、（42．72±2．80）％。处理前后杀伤率有显著性差异（F=29．05，P=0．000）。结论：苦参碱通过诱导肿瘤细胞高表达NKG2D配体（MICA/B、ULBP1-3），使肿瘤细胞对NK细胞的杀伤敏感性增强。

英文摘要：

Objective： To investigate the effect of Matrine on NKG2D ligand expression in ABCG_2^High CNE2/DDP （human multi-drug resistant nasopharyngeal carcinoma cells that highly express ATP-binding cassette superfamily G member 2） cells and to explore the mechanism of cytotoxic sensitivity to natural killer （NK） cells exerted by Matrine. Methods： ABCG2HighCNE2/DDP cells and NK cells were isolated by magnetic activated cell sorting （MACS）. Flow cytometry （FCM） was used to evaluate the purity of isolated cells and the expression of the NKG2D-ligands （NKG2D-L） on target cells before and after incubation with Matrine. Subsequently, the cytotoxic sensitivity of treated and un-treated ABCG_2^High CNE2/DDP cells to NK cells were measured by LDH releasing assay. Results： The expression of ABCG2 in ABCG_2^High CNE2/DDP cells was 91.40＋2.32%. More than 90% of isolated NK cells were shown to be CD3^-CD^16＋CD^56＋ cells which would definitely meet the needs of experiments. The expression of MICA, MICB, ULBP1, ULBP2, and ULBP3 in target cells incubated with Matrine increased from 2.92±0.33%, 4.27±0.33%, 5.80±0.62%, 11.10±3.15% and 7.75±1.14% to 11.30±0.89%, 14.29±2.61%, 12.56±1.06%, 43.24±4.43% and 12.77±1.06%, respectively. At E：T ratios of 10：1 and 20：1, the cytotoxic sensitivity of ABCG_2^High CNE2/DDP cells to NK cells increased from 15.32±1.34% and 27.26±6.81% in un-treated groups to 28.53±1.37% and 42.72±2.80% in Matrine treated groups, with a significant difference between the two groups （F=29.05, P=-0.000）. Conclusion： Matrine can upregulate the expression of NKG2D-L （MICA/B and ULBP1-3） in human multi-drug resistant nasopharyngeal carcinoma cells, resulting in higher cytotoxic sensitivity to NK cells.