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Biosurfactant-producing Ability of Highly Efficient Petroleum-degrading Bacterium BS-8
  • ISSN号:1002-1302
  • 期刊名称:《江苏农业科学》
  • 时间:0
  • 分类:S852.4[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医] TQ423[化学工程]
  • 作者机构:[1]Department of Life Sciences, Henan Institute of Education, Zhengzhou 450046, China, [2]Puyang Vocational and Technical College, Puyang 457000, China
  • 相关基金:Supported by Key Science and Technology Program of Henan Province (132102310253, 122102310350) ; Natural Science Research Program of Ed- ucation Department of Henan Province ( 15A210020, 12A180008).
中文摘要:

After incubation for 6- 30 h,with the rapid increase of bacterial cell number,surface tension of bacterium BS-8 was reduced sharply from 63. 2 m N / m to 39. 4 m N / m. The production of biosurfactants by BS-8 was growth-dependent. Using glucose as the carbon source,bacterium BS-8 was incubated. Based on centrifugation,precipitation and chromogenic reaction of the culture solution,results indicated that the biosurfactants belonged to lipopeptides. The yield of biosurfactants isolated and purified from the culture solution was 0. 58 g / L,and the critical micelle concentration( CMC) was 90 mg / L. Under conditions of p H 4- 9,temperature 20- 70 ℃,Na Cl concentration 1%- 6%,biosurfactants produced by BS-8 exhibited the highest stability.

英文摘要:

After incubation for 6 -30 h, with the rapid increase of bacterial cell number, surface tension of bacterium BS-8 was reduced sharply from 63.2 mN/m to 39.4 mN/m. The production of biosurfactants by BS-8 was growth-dependent. Using glucose as the carbon source, bacterium BS-8 was incubated. Based on centrifugation, precipitation and chromogenie reaction of the culture solution, results indicated that the biosurfactants belonged to lipopeptides. The yield of biosurfaetants isolated and purified from the culture solution was 0.58 g/L, and the critical micelle concentration (CMC) was 90 mg,/L. Under conditions of pH 4 -9, tem- perature 20 -70 ℃, NaCl concentration 1% -6%, biosurfactants predueed by BS-8 exhibited the highest stability.

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期刊信息
  • 《江苏农业科学》
  • 北大核心期刊(2011版)
  • 主管单位:江苏省农业科学院
  • 主办单位:江苏省农业科学院
  • 主编:常有宏
  • 地址:南京孝陵卫钟灵街50号
  • 邮编:210014
  • 邮箱:jsnykx@vip.163.com
  • 电话:025-84390282
  • 国际标准刊号:ISSN:1002-1302
  • 国内统一刊号:ISSN:32-1214/S
  • 邮发代号:28-10
  • 获奖情况:
  • 第二届国家期刊奖百种重点期刊奖,全国优秀科技期刊奖,江苏省“双十佳”期刊奖,国家期刊奖提名奖,华东地区优秀期刊,优秀农业期刊
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:38904