位置:成果数据库 > 期刊 > 期刊详情页
The relationship of differential expression of genes in GA biosynthesis and response pathways with heterosis of plant height in a wheat diallel cross
  • ISSN号:1002-008X
  • 期刊名称:《自然科学进展》
  • 时间:0
  • 分类:S512.1[农业科学—作物学] S512.103.5[农业科学—作物学]
  • 作者机构:[1]State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China, [2]National Plant Gene Research Centre (Beijing), Beijing 100193, China
  • 相关基金:Supported by the National Key Basic Research and DeveIopment Program of China (Grant No. 2007CB109000), and National Natural Science Foundation of China (Grant Nos. 30671297, 30600392, 30871529, 30871577)
中文摘要:

杂种优势在内部节点延伸和植物高度通常在混合植物,而是的分子的基础被观察增加内部在混血儿的节点延伸是未知的。在这研究,在植物高度的中间的父母杂种优势在包含 16 个混血儿和 8 个父母的一个小麦 diallel 十字被决定,并且即时 PCR 被用来在在混血儿和父母之间的基因表示分析改变。植物高度的重要杂种优势并且第一内部在长度的节点为所有 16 个混血儿被观察,但是杂种优势的大小为不同生气联合是可变的。分析表明杂种优势第一内部节点显著地被相关到植物高度的(r = 0.56, P 【 0.05 ) ,建议增加的延伸第一内部节点是到在植物高度的杂种优势的主要贡献者。实时 PCR 分析在基因表示的杂种优势展出了那重要差别在所有生气联合之中被观察。而且,杂种优势第一内部在长度的节点与 KS , GA3ox2-1 , GA20ox2 , GA20ox1D , GA-MYB 和 GID1-1 的表示杂种优势显著地并且断然被相关,但是显著地并且否定地与 GAI 和 GA2ox-1 的表示杂种优势,它在小麦植物高度与我们气体和杂种优势的最近建议的模型一致,建议 GA 生合成和反应小径的改变可能为在植物高度的观察杂种优势负责。

英文摘要:

Heterosis in internode elongation and plant height is commonly observed in hybrid plants, but the molecular basis for the increased internode elongation in hybrids is unknown. In this study, midparent heterosis in plant height was determined in a wheat diallel cross involving 16 hybrids and 8 parents, and real-time PCR was used to analyze alterations in gene expression between hybrids and parents. Significant heterosis of plant height and the first internode in length were observed for all 16 hybrids, but the magnitude of heterosis was variable for different cross combinations. Analysis revealed that the heterosis of the first internode was significantly correlated to that of plant height (r = 0.56, P 〈 0.05), suggesting that the increased elongation of the first internode is the major contributor to the heterosis in plant height. Real-time PCR analysis exhibited that significant difference in heterosis of gene ex- pression was observed among all cross combinations. Moreover, heterosis of the first internode in length was correlated significantly and positively with expression heterosis of KS, GA3ox2-1, GA20ox2, GA20ox1D, GA-MYB and GID1-1, but significantly and negatively with expression heterosis of GAI and GA2ox-1, which is consistent with our recently proposed model of GAs and heterosis in wheat plant height, suggesting the alteration of GA biosynthesis and response pathways might be responsible for the observed heterosis in plant height.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《自然科学进展》
  • 北大核心期刊(2008版)
  • 主管单位:国家自然科学基金委员会科学基金杂志部
  • 主办单位:国家自然科学基金委员会 中国科学院
  • 主编:师昌绪
  • 地址:北京海淀区双清路83号
  • 邮编:100085
  • 邮箱:progress@mail.nsfc.gov.cn
  • 电话:010-62326952 62327202
  • 国际标准刊号:ISSN:1002-008X
  • 国内统一刊号:ISSN:11-3852/N
  • 邮发代号:
  • 获奖情况:
  • 国家期刊提名奖,国家“双百”期刊,第二届全国优秀科技期刊评比三等奖
  • 国内外数据库收录:
  • 日本日本科学技术振兴机构数据库,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2000版)
  • 被引量:12674