中文摘要：

为进一步研究柔嫩艾美耳球虫表面抗原蛋白，应用RT—PCR从柔嫩艾美耳球虫第2代裂殖子总RNA扩增表面抗原基因SAG19（Q70CD0）和SAGfm（U6L233），与pGEM—Teasy载体连接，扩增目的片段后分别双酶切扩增产物与pET-28a（＋），连接转化至BL21后，诱导表达，分别获得两种重组蛋白。SDS—PAGE结果表明，两种重组蛋白的分子质量均为32ku，为包涵体蛋白形式。分别以感染柔嫩艾美耳球虫的鸡血清和重组蛋白免疫新西兰大白兔获得的多克隆抗体作为一抗，经Western blot分析，结果表明重组蛋白具有良好的免疫原性和抗原性。通过对这两种表面抗原基因的研究，为基因工程疫苗的研究提供一定的理论依据。

英文摘要：

In order to further study the surface antigen proteins of E. tenella, the SAG19 （U6L233） and SAGfm（QTOCD0） genes in the second generation merozoites were amplified from total RNA by RT-PCR and ligated to the pGEM-T easy vector.After the target gene fragments amplified,the two genes and pET- 28a（＋） were digested by two restriction endonucleases and then connected.The two connection products were transformed into E.coli BL21.After induction, two recombinant proteins were obtained.The expressed proteins were all inclusion bodies and the molecular mass of two recombinant proteins were all about 32 ku as revealed by analysis using SDS-PAGE.The serum of chickens infected with E.tenella and polyclonal antibodies which were produced in rabbits were served as the first antibody, and the results of Western blot indicated that the two recombinant proteins had good immunogenicity and antigenicity. The research of these two genes could provide theoretical basis for the development of genetic enginering vaccines.