通过对1,1-二苯基-2-苦肼基(DPPH)、超氧化物阴离子、羟自由基的清除试验,测定硫酸化酵母葡聚糖(s GSC)的抗氧化活性;通过测定s GSC对金黄色葡萄球菌、表皮葡萄球菌、猪链球菌、粪肠球菌、大肠杆菌、耐药金黄色葡萄球菌NY3等的最小抑菌浓度,评价s GSC体外抗菌活性。结果表明:s GSC对DPPH、超氧化物阴离子、羟自由基的清除率均随着s GSC的浓度的增加而增加,在浓度为5 mg/m L时,对3种自由基的清除率分别为89.72%、42.16%、83.41%,表明s GSC对3种自由基均有较好的清除作用,表现出了较好的抗氧化活性。s GSC对金黄色葡萄球菌、表皮葡萄球菌、猪链球菌、粪肠球菌的最小抑菌浓度均为80 mg/m L,而对大肠杆菌为40 mg/m L,表明s GSC对这几种菌具有一定的抑菌作用;而对NY3的最小抑菌浓度大于80 mg/m L,表明s GSC对耐药金黄色葡萄球菌没有抑菌作用。
Antioxidant activity of sulfated yeast glucan( s GSC) was determinated by 1,1- phenyl- 2- bitter hydrazine( DPPH),superoxide anion and hydroxyl radical scavenging tests; antibacterial activity of s GSC was determinated by the minimum inhibitory concentration( MIC) test of Staphylococcus aureus,Staphylococcus epidermidis,Streptococcus suis,Enterococcus faecalis,Escherichia coli. The results showed that s GSC could scavenge DPPH,superoxide anion and hydroxyl radical with the concentration- dependent increase. Three free radical scavenging rates in 5mg / ml were 89. 72%,42. 16% and 83. 41%,respectively,indicating that s GSC had good antioxidant activity.MICs of s SGSC were 80 mg / ml for S. aureus,S. epidermidis,S. suis and E. faecalis,and 40 mg / ml for E. coli,suggesting that s GSC had antibacterial effects against these bacteria. However,s GSC had no inhibitory effects against drug- resistant S. aureus with MIC of more than80 mg / ml.