中文摘要：

目的观察黄芩苷对低氧性人肺动脉平滑肌细胞（HPASMCs）增殖的干预作用，并比较国产和进口来源黄芩苷的药效差异。方法免疫荧光化学技术鉴定HPASMCs，在低氧条件下建立HPASMCs过度增殖模型，采用MTT法分别检测常氧和低氧环境中不同剂量国产与进口来源黄芩苷对HPASMCs增殖的影响。结果24b开始低氧即可促进HPASMCs增殖，但此时黄芩苷干预对其无明显影响，而经48h处理后HPASMCs被显著抑制，分别以终浓度5、10、20、40μmol／L国产或进口黄芩苷处理异常增殖的HPASMCs，发现10μmol／L国产黄芩苷和40μmol／L进口黄芩苷表现出最佳的抑制效果，而从综合比较和药效程度而言以40μmol／L进口黄芩苷效果更显著（P〈0．01）。结论黄芩苷能有效抑制HPASMCs增殖，且进口黄芩苷的干预作用相对较强。

英文摘要：

Objective To compare the effects of imported baicalin with domestic baicalin on hypoxic proliferation of human pulmonary arterial smooth muscle cells （HPASMCs） and to reveal the effects of baicalin intervention in this condition. Methods Immunofluorescence was used to identify the HPASMCs cultured in vitro. A hyperproliferation model of HAPSMCs was established under hypoxic condition. The proliferation of HPASMCs treated with either imported baicalin or domestic baicalin under normoxia and hypoxia was measured by the MTT assay, Results Hypoxia can induce HPASMC proliferation after 24 h, when baicalin intervention did not affect HPASMC growth. At 48 h after treatment, both 10μmol/ L of domestic baicalin and 40 μmol/L of imported baicalin significantly inhibited the HPASMC proliferation induced by hypoxia. However, the inhibitory effect of imported baicalin was better （ P 〈 0. 01 ）. Conclusion Baicalin has inhibitory effects on HPASMC proliferation. The imported baicalin has stronger effects.