中文摘要：

目的：探讨盐酸小檗碱体外抗单纯疱疹病毒1型（HSV-1）的作用及作用机制。方法：MTT法检测盐酸小檗碱对Vero细胞的毒性作用,观察HSV-1感染Vero细胞后所致的细胞病变效应（Cytopathologic effects,CPE）,实时荧光定量PCR法检测盐酸小檗碱对病毒蛋白gD的mRNA表达水平,免疫印迹（Western blot）法检测盐酸小檗碱对病毒蛋白ICP27和ICP8表达的影响。结果：盐酸小檗碱半数中毒浓度（TC50）为392.5μmol/L,病毒半数抑制浓度（IC50）为66.49μmol/L,选择性指数（SI）为5.9。实时荧光定量PCR结果显示盐酸小檗碱可降低HSV-1 gD的mRNA表达水平。Western blot显示盐酸小檗碱可明显抑制病毒蛋白ICP27和ICP8的表达。结论：盐酸小檗碱具有抗HSV-1的作用,可能与抑制病毒蛋白ICP27的表达进而影响ICP8和gD的表达有关。

英文摘要：

Objective： To study the antiviral activity and mechanism of Berberine against herpes simplex virus 1（ HSV-1） in vitro.Methods： MTT assay was adopted to evaluate the cytotoxicity of Berberine.The cytopathologic effects（ CPE） that HSV-1 infected Vero cells was observed.Quantitative real-time PCR assay was conducted to determine mRNA expression of late protein g D relating to viral replication.Western blot assay researched the expression of viral proteins ICP27 and ICP8.Results： The median toxic concentration（ TC50） of Berberine was 392.5μmol/L,median inhibition concentration（ IC50） was 66.49 μmol/L,and selectivity index（ SI） was 5.9.Quantitative real-time PCR assay showed that the expression of viral protein g D mRNA was inhibited by Berberine.Western blot revealed that the expressions of viral proteins ICP27 and ICP8 were obviously inhibited by Bererine.Conclusion： It demonstrated that Berberine has antiviral activity against HSV-1 in vitro,which was maybe related to inhibiting the expression of ICP27 protein.Subsequently,the inhibition of ICP27 protein affects the expression of ICP8 and g D.