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Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca~(2+)-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats
  • ISSN号:1007-9327
  • 期刊名称:《世界胃肠病学杂志:英文版》
  • 时间:0
  • 分类:R657.51[医药卫生—临床医学;医药卫生—外科学]
  • 作者机构:[1]Departnaent of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China, [2]Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • 相关基金:The National Natural Science Foundation of China, No. 30500684; the Medical Science and Technology Research Foundation of State Administration of Traditional Chinese Medicine, No. 04-05JP55.
中文摘要:

瞄准:在尖锐胰腺炎(AP ) 在胰腺的纸巾的 sarco/endoplasmic 蜂窝胃 Ca2+-ATPase (SERCA ) mRNA 表示上调查 Chaiqinchengqi 煎(CQCQD ) 的效果老鼠。方法:三十只 Sprague-Dawley (SD ) 老鼠被使随机化进控制组, AP 组和 CQCQD 组(3 乘的 n = 10 ) 。在 CQCQD 组的老鼠 intragastrically 与 CQCQD 被管理(10 mL/kg 每 2 h ) 在由 caerulein 的腹膜内注射的 AP 的正式就职以后(50 mug/kg.h 乘 5 ) 在 4 h 以内。在在 AP 模型的正式就职以后的 6 h,胰腺的纸巾为病理学的观察被收集,为为细胞内部的钙离子集中的荧光紧张(FI ) 的测量的 SERCA1 和 SERCA2 mRNA 表示或胰腺的腺泡房间隔离的决心的 mRNA 抽取[Ca2+] 我。结果:没有在控制组和 AP 组的胰腺的 SERCA1 mRNA 的表示。在 AP 组的胰腺的 SERCA2 mRNA 的表示是下面调整的(表示比率 = 0.536;P = 0.001 ) 与控制组相比,当那在 CQCQD 组是起来调整的时(表达式比率 = 2.00;P = 0.012 ) 与 AP 相比组。 FI 细胞内部[Ca2+]胰腺的腺泡,在 AP 的房间组织( 138.2 +/- 23.1 )比 C 组高( 111.0 +/- 18.4 )并且 CQCQD 组( 118.7 +/- 15.2 )( P 【 0.05 )并且在 CQCQD 组的胰腺的病理学的分数更低在 AP 比那组织( 5.7 +/- 1.9 对 9.2 +/- 2.7 , P 【 0.05 )。结论:CQCQD 罐头起来调整胰腺的纸巾的 SERCA2 mRNA 的表示,减少细胞内部的钙超载并且减轻胰腺的织物损害。

英文摘要:

AIM: To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. METHODS: Thirty Sprague-Dawley (SD) rats were randomized into control group, AP group and CQCQD group (n = 3 × 10). The rats in the CQCQD group were intragastrically administered with CQCQD (10 mL/kg every 2 h) after induction of AP by intraperitoneal injection of caerulein (50 μg/kg.h × 5) within 4 h. At 6 h after the induction of AP model, pancreatic tissues were collected for the pathological observation, mRNA extraction for determination of SERCA1 and SERCA2 mRNA expression or pancreatic acinar cell isolation for measurement of fluorescence intensity (FI) of intracellular calcium ion concentration [Ca2+ i. RESULTS: There was no expression of pancreatic SERCA1 mRNA in the control group and the AP group. The expression of pancreatic SERCA2 mRNA in the AP group was down-regulated (expression ratio = 0.536; P = 0.001) compared with the control group, while that in the CQCQD group was up-regulated (expression ratio= 2.00; P = 0.012) compared with AP group. The FI of intracellular [Ca2+ of pancreatic acinar cells in the AP group (138.2 ± 23.1) was higher than the C group (111.0 ± 18.4) and the CQCQD group (118.7 ± 15.2 ) (P 〈 0.05) and the pancreatic pathological score in the CQCQD group was lower than that in the AP group (5.7 ± 1.9 vs 9.2 ± 2.7, P 〈 0.05).CONCLUSION: CQCQD can up-regulate the expression of SERCA2 mRNA of pancreatic tissues, reduce intracellular calcium overload and relieve pancreatic tissue lesions.

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  • 《世界胃肠病学杂志:英文版》
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  • 国际标准刊号:ISSN:1007-9327
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