中文摘要：

目的探讨心肌营养素-1（CT-1）在窒息新生大鼠心肌和外周血血浆中的表达,以及神经调节蛋白-1（NRG-1）的干预作用。方法新生7日龄大鼠90只,随机分成窒息组（n=40）、正常对照组（n=10）和NRG-1预处理组（n=40）。采用颈动脉结扎联合缺氧法制备新生大鼠窒息模型。NRG-1预处理组在缺氧缺血前30min腹腔注射1mg/kgNRG-1。分别在造模后6、12、24、48h各时间点取其外周血和左心室前壁心肌,酶联免疫吸附法检测其外周血血浆CT-1蛋白的表达。采用Western blot检测其心肌CT-1蛋白表达。结果窒息组新生大鼠外周血血浆CT-1的表达在各时间点均显著高于正常对照组,24h达到高峰（Pa〈0.05）;窒息后24h,新生大鼠外周血血浆CT-1的表达与CK-MB水平呈显著正相关（r=0.733P〈0.01）。窒息后新生大鼠心肌中CT-1的表达逐渐增加,在各时间点均显著高于正常对照组（Pa〈0.05）。其心肌中CT-1蛋白表达量在24h达高峰（4176.5±341.6）,显著高于正常对照组（1211.9±237.7）（P〈0.05）;窒息后24h,NRG-1预处理组外周血血浆CT-1蛋白表达[（1287.7±18.9）ng/L]显著高于窒息组[（712.5±24.6）ng/L]（P〈0.05）。结论窒息可导致新生大鼠心肌和外周血血浆CT-1的表达增加。CT-1可能为心肌细胞损害的诊断提供一个新的检测指标。NRG-1可通过诱导CT-1表达上调,产生保护心肌的作用。

英文摘要：

Objective To explore the expression of cardiotrophin - 1 （CT - 1 ） in myocardium and peripheral blood plasma of neonatal rat with asphyxia and the regulative effect of neuregulin - 1 （ NRG - 1 ）. Methods Ninety seven - day - old neonatal rats were randomly di- vided into 3 groups：asphyxia group （n = 40）, normal control group （n = 10）and NRG - 1 pretreatment group （n = dO）. The model of neonatal rat with asphyxia was prepared by the way of ligation of carotid combined with low supply of oxygen. NRG - 1 （ 1 mg/kg） was given to NRG - 1 pretreatment group by intraperitoneal injection 30 min before asphyxia. The separated plasma of peripheral blood and myocardium antetheca of aortic ventricle of heart were taken at the time point of 6,12,24 and 48 h. The expression of CT - 1 in peripheral blood plasma was detected by enzyme linked immunoadsorbent assay,and that of myocardium was determined by Western blot. Results The expressions of CT - 1 pro- tein in peripheral blood plasma of asphyxia group were significantly higher than those of normal control group at each time point, and reached the peak at 24 h （P. 〈 0.05 ）. After 24 h of asphyxia, the expressions of CT - 1 were directly correlated with the level of CK - MB in periphe- ral blood plasma of neonatal rat with asphyxia （ r = 0.733 P 〈 0.01 ）. The expressions of CT - 1 in myocardium were gradually increased after asphyxia, which were markedly higher than those of normal control group at each time point （ Pa 〈 0.05 ）. The expressions of CT - 1 protein in myocardium of neonatal rats with asphyxia reached the peak at 24 h（4 176.5 ± 341.6） ,which were significantly higher than those of normal control （ 1 211.9 ± 237.7 ） （ P 〈 0.05 ）. After 24 h of asphyxia, the level of CT - 1 protein [ （ 1 287.7 ± 18.9） ng/L ] in peripheral blood plas- ma of NRG - 1 pretreatment group were higher than those of asphyxia group [ （ 712.5 ± 24.6 ） ng/L ] （ P 〈 0.05 ）. Conclusions Asphyxia can induce the increase of