中文摘要：

目的：探讨血管生成素在小鼠皮肤中的表达及其对小鼠毛发生长的影响。方法：利用松香一石蜡拔毛法诱导C57BL／6小鼠背部的毛囊同步进入生长期，半定量RT-PCR检测血管生成素mRNA在不同毛囊周期小鼠皮肤中的表达。分离与培养完整的小鼠触须毛囊，加入不同浓度的血管生成素（0～200斗g，L），培养8d后测量毛囊的生长长度。与此同时，皮内注射血管生成素或血管生成素真核表达质粒pEGFP-ANG，并设立同时注射抗人血管生成素抗体组、注射空质粒pEGFP-C2组和注射赋形剂的对照组，观察小鼠局部皮肤颜色的改变，并借助苏木精一伊红染色组织切片测量皮肤的厚度和计数生长期Ⅵ毛囊的比例。结果：半定量RT-PCR显示小鼠皮肤中血管生成素mRNA在生长期表达逐渐增加，生长期晚期表达最高，退行期开始减少，至休止期表达最低，其表达模式与毛囊周围的血管形成相一致。12．5～200．0斗g／L血管生成素呈剂量依赖性地促进体外培养的小鼠触须毛囊生长（P〈0．01）。而且，皮内注射血管生成素和血管生成素真核表达质粒对C57BL／6小鼠背部的局部皮肤颜色无明显影响，但能明显增加局部皮肤的厚度（P〈0．01）和生长期Ⅵ毛囊的比例（P〈0．01）。结论：血管生成素在小鼠皮肤中的表达具有毛囊周期依赖性的特点．血管生成素可能通过促进毛囊周围的血管形成和诱导毛囊进入生长期Ⅵ而促进小鼠毛发的生长。

英文摘要：

Objective： To investigate the expression of angiogenin in the mouse skin of different hair cycles and its role in promoting the hair growth. Methods： Anagen was induced by melted wax/rosin on the back skin of the mouse in the telogen phase, followed by semi-quantitative RT-PCR to assess angiogenin mRNA expression in different hair cycles. Mouse vibrissa follicles were cultured with different concentrations of angiogenin （0～200.0 txg/L） for 8 days and the length of hair follicle grown was measured. In addition, pEGFP-ANG or angiogenin was injected intradermally into C57BL/6 mice. Injections of anti-human angiogenin antibody,empty pEGFPANG and vehicle served as controls. In addition to assessing the changes of skin color and the skin thickness, the percentage of hair follicles in anagen VI was evaluated microscopically. Results： The expression of angiogenin mRNA in the mouse skin increased with a peak in late anagen and declined in the catagen phase, reaching the lowest levels in the telogen phase. This expression pattern was consistent with that of the perifollicular angiogenesis. Angiogenin in the concentrations of 12.5-200.0 wg/L significantly stimulated the growth of cultured mouse vibrissa follicles in a dose-dependent manner（P〈 0.01）. Skin color of angiogenin or pEGFP-ANG injected sites did not differ significantly from the controls. Histologically, the angiogenin and pEGFP-ANG injected sites of the skin showed a significant in- crease in both the thickness （P 〈 0.01） and the percentage of hair follicles in anagen VI （P 〈 0.01）. Conclusions： The expression of angiogenin mRNA in mouse skin is in a hair-cycle dependent manner, with maximum levels at the late anagen, and angiogenin could stimulates the mouse hair growth by promoting the perifollicular angiogenesis and inducing the hair follicles entering into anagen VI.