中文摘要：

目的：基于大鼠心肾综合征（cardiorenal syndrome,CRS）模型,探讨参附强心丸对CRS大鼠心肾部位Bcl-2/Bax蛋白表达及抗凋亡的作用机制。方法：采用腹主动脉缩窄合并肾脏急性缺血再灌注致大鼠心肾综合征模型,大鼠随机分为假手术对照组、模型对照组、参附强心丸高、中、低剂量组、卡托普利对照组6组,给药4周后测定血液中脑钠肽、醛固酮、肌酐等,计算左肾、心室脏器指数,TUNEL法测定心、肾凋亡,免疫组化测定心、肾Bax、Bcl-2蛋白表达。结果：模型组大鼠血浆脑钠肽、醛固酮、肌酐、心室指数明显高于假手术组,肾素活性、左肾指数明显降低,组织凋亡明显。参附强心丸中剂量组脑钠肽、肌酐降低明显;高剂量组醛固酮明显降低,可明显改善心肌肥厚及左肾坏死萎缩状态,上调大鼠心肾组织Bcl-2表达,抑制Bax表达,降低心、肾细胞凋亡率。结论：参附强心丸高、中剂量组可以改善CRS大鼠肾功能、水钠潴留状态,通过上调Bcl-2表达,抑制Bax表达,降低心肾组织凋亡,起到对心肾的保护作用。

英文摘要：

Objective： Based on the cardiorenal syndrome rats model,to explore Shenfu Qiangxin（SFQX） pills of CRS in rat heart and kidney site of Bcl-2/Bax protein expression and anti apoptosis mechanism. Methods： A rat model of CRS was induced by ACC ＋ RIRI. After operation,the CRS rats were randomly divided into 6 groups： Sham group,CRS model group,SFQX pills high、medium、low group and captopril group,Gavage rats 4 weeks,testing rats of BNP,ALD,Cr etc. Computed ventricular,heart,left renal index; Apoptosis of myocardium and kidney were measured by TUNEL and Bax and Bcl-2 protein expression in myocardium and kidney were measured by immunohistochemistry.Results： The CRS group BNP,Bun,Cr,ALD,ventricular index were higher than that of the sham group,PRA,AT1 and AT2,left renal index was significantly decreased,left ventricular and left renal tissue apoptosis. SFQX pills of BNP and Cr obviously decrease,can improve the low renin status of CRS rats; high group ALD decreased significantly;significantly improve myocardial hypertrophy,improve atrophy of left kidney necrosis,tune of CRS rats myocardial and kidney Bcl-2 expression and inhibition of Bax expression,reduce myocardial and renal cell apoptosis rate. Conclusion：SFQX pills can improve CRS rats renal function,sodium and water retention,by up regulating Bcl-2 protein expression and inhibiting the expression of Bax,reduce apoptosis of heart and kidney tissues to have a protective effect on the heart and kidney.