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NDAP（神经元发育相关蛋白）对发育过程中凋亡的调控作用
  • 项目名称：NDAP（神经元发育相关蛋白）对发育过程中凋亡的调控作用
  • 项目类别：面上项目
  • 批准号：30670644
  • 申请代码：C090201
  • 项目来源：国家自然科学基金
  • 研究期限：2007-01-01-2009-12-31
  • 项目负责人：于常海
  • 负责人职称：教授
  • 依托单位：北京大学
  • 批准年度：2006
中文摘要：

神经元发育相关蛋白（neuronal development-associated protein，NDAP)是本实验室在原代培养的小鼠皮层神经元模型中，利用RNA指纹图谱技术克隆并鉴定的新蛋白。在已结题的国家自然科学基金面上项目中，我们在基因水平证实NDAP与神经元发育有明显相关性并受神经营养因子调节，且高水平的NDAP可以引起细胞凋亡。本项目中我们对NDAP蛋白水平的表达、分布和调控机制进行了研究，并初步探讨了NDAP引起细胞凋亡的机制。我们制备了NDAP的多克隆抗体，并将该抗体应用于对NDAP蛋白水平的研究。结果显示发育过程中，NDAP蛋白水平在原代培养的神经元、星形胶质细胞和脑组织中存在差异性表达，其表达水平受神经营养因子调控，与基因水平的研究结果相一致。我们发现高表达的NDAP会引起内质网应激相关分子Bip、ATF-4和CHOP的增加，提示NDAP通过Bip-ATF-4-CHOP通路引起内质网应激，其在内质网的升高可能是内质网蛋白参与发育凋亡筛选的重要机制之一。本项目对于NDAP的研究将有利于深入认识神经营养因子调控下的发育凋亡机制，拓展内质网蛋白的功能研究，有重要科学价值。

中文主题词： NDAP；神经发育；神经营养因子；内质网应激；凋亡
结论摘要：

英文主题词neuronal development-associated protein; neuronal development; neurotrophin; endoplasmic reticulum stress; apoptosis

成果综合统计
成果类型
数量
  • 期刊论文
  • 会议论文
  • 专利
  • 获奖
  • 著作
  • 25
  • 56
  • 0
  • 6
  • 1
期刊论文
脑缺血性疾病中星形胶质细胞保护机制研究
FAAP, a novel murine protein, is involved in cell adhesion through regulating vinculin-paxillin asso
Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA)
A tribute to Professor Ji-Sheng Han
Single-ensemble-based eigen-processing methods for color flow imaging--Part II. The matrix pencil es
Single-ensemble-based eigen-processing methods for color flow imaging--Part I. The Hankel-SVD filter
[Progress in functional polyphosphate in prokaryotic and eukaryotic living organisms]
The Third ISN Special Neurochemistry Conference--8th International Meeting on Brain Energy Metabolis
Detection of Newcastle disease virus using nucleic acid sequence-based amplification
H5N1 infection of the respiratory tract and beyond: a molecular pathology study
Sinomenine inhibits activation of rat retinal microglia induced by advanced glycation end products
AGEs mediated expression and secretion of TNF alpha in rat retinal microglia
Glial cell line-derived neurotrophic factor protects astrocytes from staurosporine- and ischemia- in
The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elon
多聚磷酸盐在原核和真核生物中的研究进展
异常活化的小胶质细胞的特征与功能
会议论文
星形胶质细胞新进展及其在临床上的应用前景
Glutamate induces astrocytic nuclear swelling specifically through aquaporin-1 in nuclear membrane
The hyperpolarization-activated nucleotide-gated channel (HCN) in astrocyte regulated by glutamate a
Functional NMDA receptor induced by ischemia in astrocytes
Astrocytes play a role in chronic pain maintenance through DREAM and dynorphin
Glutamate uptake triggers astrocytic nuclear swelling through aquaporin-1 located on nuclear membran
Consultation on diagnosis of H5N1 avian influenza infections in humans
Astrocytes perform endocytosis to become hypertrophic
Decrease inorganic polyphosphate by PPX transfection in C6 rat glioma cell line could change its res
Decrease inorganic polyphosphate by PPX transfection in C6 rat glioma cell line could change its res
慢行疼痛治疗的新靶点：星形胶质细胞
Novel PCR-based pathogen detection system for rural areas
Glutamate induces nuclear swelling in astrocyte through aquaporin-1
NDAP appears to cause apoptosis and localize in endoplasamic reticulum
Uptake of glutamate into astrocytic nucleus measured by RP-HPLC
DREAM distribution in astrocyte, GABAergic neuron and glutamatergic neuron
Endogenous neuroprotective protein to protect cell death under ischemia
Neuroglobin but not cytoglobin binds specifically to mitochondria in neural cells.
DREAM is expressed in primary culture of astrocyte and can be regulated by substance P, bradykinin a
Difference distribution of DREAM/calsenilin/KChIP 3 in cultured cortical neuron and astrocyte
Glutamate directly triggers nuclear swelling in astrocyte through aquaporin-1
The importance of molecular diagnosis for avian influenza viruses
Ischemia caused JNK activation to induce 14-3-3 γ up-regulation in astrocyte.
Glutamate and ammonia induce cell and nuclear swelling in cultured astrocyte
Functional dynorphin is present in cultured astrocytes
Ischemia induced functional NMDA receptors in astrocytes.
Establishment of a cell line with stable expression of exopolyphosphatase (PPX).
Enabling growth of quality clinical in vitro diagnostics (IVDs) – Keynote Presentation: Diagnostics
Protein level analysis of NDAP, the neuronal development-associated protein.
Biotechnology in Hong Kong - Hai Kang Life Corporation from innovation to commercialization
星形胶质细胞参与慢性痛的机制
Expression of functional hyperpolarization-activated channels (HCN channels) in mouse cortical astro
Comparison of different methods for quantification of inorganic polyphosphate.
How new, efficient but inexpensive biotechnological techniques cold be able to assist local islander
Winning the war against influenza pandemics: The demand for innovative dignostic tools for pre-empti
Novel field-assisted lab-on-a-chip system for rapid point of care diagnosis
Are we prepared for the battle against influenza?
Early detection of H5N1 and SARS virus
Novel nucleic acid-based lab-on-a-chip for infectious disease diagnosis
Rapid point-of-care diagnosis using novel field-assisted lab-on-a-chip system
Novel field-assisted lab-on-a-chip system for rapid point-of-care diagnosis
强啡肽原及其转录调节因子DREAM在星形胶质细胞中的表达和调节
Intrinsic protective protein: factors to protect us from neural injury
Astrocytes in primary culture as a cell model to study CNS injury and ageing
Glutamate induces a specific nuclear size change in astrocyte through aquaporin-1
14-3-3 γ could be up-regulated through JNK pathway to protect cell death in astrocyte under ischemia
14-3-3 γ could be up-regulated through JNK pathway to protect cell death in astrocyte under ischemia
Collaborative opportunities on bioInformatics in the diagnosis of infectious diseases:Our preparedne
Ischemia up-regulates 14-3-3 γ in astrocyte through phosphorylation of c-Jun
Decrease inorganic polyphosphate by PPX transfection in C6 rat glioma cell line could change its res
Astrocytic nuclear swelling in respond to extracellular glutamate
Quantitative measurement of glutamate uptake by isocratic reversed high performance liquid chromatog
Astrocytes expresses HCN channel which responds to K+ and glutamate.
Active nuclear swelling induced by glutamate and ammonia in cultured astrocyte.
脑缺血性疾病中星形胶质细胞保护机制的研究
Glutamate modulates shape of astrocytic nucleus through aquaporin-1
获奖
神经生物学疼痛专题PBL与LBL相结合的教学探索
神经生物学疼痛专题PBL与LBL相结合的教学探索
北京大学医学部优秀人才奖励计划
亚洲知识管理奖
2008年度北京大学医学部优秀教师
基于NASBA技术的传染病（禽流感）早期诊断平台
著作
Neurochemical Research: Special Issue Dedicated to Professor Ji-Sheng Han
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