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中文摘要：
本项目瞄准国际上P450与昆虫抗药性关系这一研究热点，以目前粮仓中严重危害的书虱类害虫嗜卷书虱为研究对象，综合运用昆虫生理生化和分子生物等学科知识，借助相关最新的分子生物学研究手段，利用细胞色素P450的可诱导性，比较研究书虱敏感品系、抗性品系及诱导品系对有机磷类常用杀虫剂及PH3的敏感性差异；应用RT-PCR及RACE技术克隆书虱P450基因的全长序列，结合Northern杂交和RT-qPCR技术解析其mRNA在嗜卷书虱不同发育历期和不同品系的表达模式；通过不同品系P450等位基因的序列比对进而找出与抗性相关的突变位点；通过P450的异源表达并分析表达产物（酶）的酶学特性；最终阐明P450介导书虱代谢抗性的分子生物学机制。最后应用PCR-RFLP及RT-qPCR技术建立自然种群抗性的分子诊断技术。
结论摘要：
英文主题词Liposcelis bostrychophila; P450; gene cloning; expression; resistance diagnosis
成果综合统计
期刊论文
会议论文
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期刊论文
Comparative studies of acetylchoninesterase purified from three field populations of Liposcelis ento
Characterization of the purified glutathione S-transferases from two psocids Liposcelis bostrychophi
Cloning and characterization of acetylcholinesterase 1 genes from insecticide-resistant field popula
Validation of endogenous reference genes for insecticide-induced and developmental expression profil
Molecular characterization of two novel deltamethrin-inducible P450 genes from Liposcelis bostrychop
Influence of continuous high temperature conditions on Wolbachia infection frequency and the fitness
Molecular characterization of two nocotinic acetylcholine receptor subunits from Liposcelis bostrych
Purification and biochemical characterization of glutathione S-transferases from three strains of Li
Purification and partial characterization of glutathione S-Transferase from insecticide-resistant fi
Development, survival, and reproduction of the psocid Liposcelis yunnaniensis (Psocoptera: Liposceli
会议论文
Partial characterization of glutathione S-transferases from different field populations of Liposceli
Cloning and evaluation of reference genes for gene expression study using real-time PCR in the psoci
Construction and identification of enriched microsatellite library from Liposcelis bostrychophila (P
Partial characterization of purified glutathione S-transferases from Liposcelis bostrychophila (Psoc
Cloning and expression of a beta-actin gene from Liposcelis bostrychophila Badonnel (Psocoptera: Lip
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