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洋葱新病原细菌Burkholderia cenocepacia的致病基因及检测技术研究
  • 项目名称：洋葱新病原细菌Burkholderia cenocepacia的致病基因及检测技术研究
  • 项目类别：面上项目
  • 批准号：30871655
  • 申请代码：C1407
  • 项目来源：国家自然科学基金
  • 研究期限：2009-01-01-2011-12-31
  • 项目负责人：谢关林
  • 负责人职称：教授
  • 依托单位：浙江大学
  • 批准年度：2008
中文摘要：

本项目的前期研究中有两个首次报道的重要发现一是发现Burkholderia cenocepacia不但是引起人体"洋葱伯克氏综合症"的中国医院优势基因型，而且也是引起洋葱腐烂的病原；二是从引发洋葱腐烂的B. cenocepacia 菌中克隆到致病基因。本研究拟通过对该种内不同类型菌株的致病性、III型分泌系统泌出性测定及运用抑制消减杂交、标签插入突变和基因敲除等技术寻找和鉴定、检测它们的致病基因，通过对人、植致病菌的比较,明确它们在致病基因上的异同,试图获得人植共患病的可靠证据,建立首个洋葱伯克氏菌致病基因数据库及B. cenocepacia B型菌检测技术。这在国内外植物病原细菌研究中尚属首次。该项目的完成可望在植物病害的理论研究上有新的提升, 为国内外同类研究开创新的视点，同时为植检部门对危险性病原细菌B. cenocepacia B型菌的检测提供具有自主知识产权的关键性技术。

中文主题词： 洋葱伯克氏菌基因型III；致病基因；人植共患病原；B. cenocepacia B型菌；检测
成果综合统计
成果类型
数量
  • 期刊论文
  • 会议论文
  • 专利
  • 获奖
  • 著作
  • 38
  • 32
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  • 0
期刊论文
Bacterial brown stripe of rice in soil-less culture system caused 3 by Acidovorax avenae subsp. aven
Association of a Bacillus species with leaf and twig dieback of Asian pear (Pyrus pyrifolia) in Chin
Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot patho
Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm
Genome sequence of the rice pathogenic bacterium Acidovorax avenae subsp. avenae RS-1.J Bacteriology
Characterization and Inference of Gene Gain/Loss Along Burkholderia Evolutionary History. Evolutiona
Effects of Paenibacillus strains and chitosan on plant growth promotion and control of Ralstonia wil
Real time fluorescence PCR method for detection of Burkholderia glumae from Rice.
Diversity Analysis of Burkholderia cepacia Complex in the Water Bodies of West Lake, Hangzhou, China
Phenotypic and molecular characterization of rhizobacterium Burkholderia sp. strain R456 antagonisti
Genome Sequence of the Enterobacter mori Type Strain, LMG 25706, a Pathogenic Bacterium of Morus alb
Diversity of Burkholderia cepacia Complex from the Moso Bamboo (Phyllostachys edulis) Rhizhosphere S
Horizontal gene transfer in silkworm, Bombyx mori.
The salicylate-containing Pseudomonas putida antibiotic promysalin promotes surface colonization and
Copper as an antibacterial agent for human pathogenic multidrug resistant Burkholderia cepacia compl
Prevelence of potential pathogenicity and molecular characterizations of Burkhoderia cepacia complex
Specific and sensitive detection of Enterobacter morus using reliable RT-PCR reaction.
Enterobacter mori sp. nov., a novel Enterobacter species associated with bacterial wilt on Morus alb
Reclassification of Xanthomonas Isolates Causing Bacterial Leaf Spot of Euphorbia pulcherrima.
Genome sequence of Stenotrophomonas maltophilia RR-10 isolated as an endophyte from rice root
Characterization of Burkholderia cepacia complex from cystic fibrosis patients in China and their ch
Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of A
Grain Discoloration of Rice Caused by Pantoea ananatis (synonym Erwinia uredovora) in China.
抗不同青枯菌生化型生防菌的筛选鉴定及其活性分析.
水稻细菌性谷枯病菌的实时荧光PCR检测技术
Enterobacter spp. : 引起“桑枯萎”病的新证据.
Effect of chitosan solution on the bacterial septicemia disease of Bombyx mori (Lepidoptera: Bombyci
Enterobacter spp.: A new evidence causing bacterial wilt on mulberry.
Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt d
Effect of Chitosan Solution on the Inhibition of Pseudomonas fluorescens Causing Bacterial Head Rot
梨火疫病病原菌（Erwinia amylovora）三型分泌系统的鉴别及Erwinia spp. HrpA 的分析.
First?Report?of?Bacterial?Head?Rot?of?Broccoli?Caused?by?Pseudomonas?fluorescens?in?China.
Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China.
洋葱伯克氏菌群不同基因型菌株对几种重要植物病原真菌的抑制作用及其潜在致病性.
梨火疫病病原菌（Erwinia amylovora）三型分泌系统的鉴别及Erwinia spp. HrpA的分析
青枯病和根肿病生防细菌BS2004的发酵培养基和培养条件优化
浙江省红掌细菌性疫病病原鉴定
会议论文
Enterobacter spp. : A new evidence causing bacterial wilt on mulberry
Diversity and distribution of Burkholderia cepacia complex from the bamboo rizhosphere
洋葱伯克氏菌的利用及其风险
桑树 “凋萎”症状的诊断及其病原检测研究
A New bacterial diseases of Mulberry related to bacterial wilt
桑树新病原—细菌性枯萎病原研究
Biological control of bacterial wilt by Pseudomonas fluorescens F091
Development of specific primers for rapid identification of Ralstonia solanacearum (host Morus alba
CHARACTERIZATION OF SELECTIVE ANTIBACTERIAL COMPOUNDS PRODUCED BY RHIZOSPHERE PSEUDOMONAS
肠杆菌科细菌的水平转移研究
洋葱伯克氏菌引起的杏果腐病病原研究
洋葱伯克氏细菌在苜蓿模型上的致病性研究
植物病原细菌与生防细菌生物膜形成的因子研究
生物膜在植物病原细菌与生防细菌中的功能研究
基因水平转移在植物细菌致病性及其进化上的影响研究
Real Time Fluorescence PCR Method for Detection of Burkhoderia glumae from Rice
Genotypes of Burkholderia cepacia complex from the patients in China and their chitosan susceptibili
Novel antimicrobial compounds produced by rhizosphere Pseudomonas
Genomic sequence of Acidovorax avenae subsp. avenae from rice
Prevalence Of Potential Pathogenicity and Molecular Characterizations Among Isolates of Burkhoderia
洋葱伯克氏菌菌中的植物检疫性病害
青枯病生防菌株BS2004抑菌物质产生条件的优化
Prevalence Of Potential Pathogenicity and Molecular Characterizations Among Isolates of Burkhoderia
Burkholderia cepacia complex on rice seeds
Utilization of Burkholderia cepacia complex and their risk in Agriculture
GENOMOVARS of BURKHOLDERIA CEPACIA COMPLEX FROM RICE RHIZOSPHERE AND CLINIC IN CHINA
Detection of Burkholderia glumae from Rice by Real-time Fluorescence PCR
桑树细菌性枯萎病病原菌Enterobacter sp.绿色荧光蛋白基因转化
桑细菌性枯萎病菌的rep-PCR、全细胞脂肪酸及致病能力多态性分析
Molecular characterization of antimicrobial compounds produced by rhizosphere Pseudomonas
洋葱伯克氏菌作为生物农药的利用及其风险
Some strains of Burkholderia cepacia complex as Biocontrol agents
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