目的:建立石芽茶中山茶苷A的含量测定方法.方法:采用反相高效液相法,以XB-C18柱(4.6 mm×250mm,5μm)为分离柱,以乙腈-0.1%磷酸(20:80)为流动相,检测波长为330nm,柱温为室温.结果:山茶苷A在(20.2~323.2)μg/ml范围内,峰面积与其浓度呈良好线性关系(r=1.000),平均回收率为98.8%,RSD为1.62%(n=6).结论:该方法简便、准确、重复性好,可作为该药材含量测定方法.
Objeetive To set up determination the contents of Camelliagenin A in Adinandra nitida. Methods RP - HPLC method was used, Chromatographic condition included XB- C18 (4. 6mm × 250mm, 5 (m) and the mobile phase consisting of acetonitrile -0. 1% phosphoric acid (20: 80) , with a detection wavelength of 330nm and the temperature to room temperature. Result The cal- ibration curves were linear in the range of 20. 2 - 323.2μg,/ml for Camelliagenin A, r = 1. 000, average recovery of Camelliagenin A was 98. 8%, RSD was 1.62% (n =6). Conclusions The method is simple, accurate and repeatable, which applied in determina- tion of Camelliagenin A in Adinandra nitida.