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新疆冬春麦区小麦地方品种贮藏蛋白遗传多样性研究
  • ISSN号:1672-1810
  • 期刊名称:《植物遗传资源学报》
  • 时间:0
  • 分类:Q943[生物学—植物学] S831.8[农业科学—畜牧学;农业科学—畜牧兽医]
  • 作者机构:[1]西北农林科技大学农学院,陕西杨凌712100
  • 相关基金:西部地区特色植物种质资源数据平台建设项目(505016)
中文摘要:

采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)对236份新疆小麦地方品种的高分子量麦谷蛋白亚基(HMW-GS)的组成进行了分析。结果表明:Glu-Ⅰ位点共有19种等位基因,其中Glu-Al位点3种,Glu-Bl位点7种,Glu—D1住点9种;亚基null、7+8、2+12在各自的位点上出现频率最高,分别达到91.95%、85.17%、80.93%;亚基组成类型共有21种,主要为null/7+8/2+12,频率达70.34%;同时筛选出33份含有1、2^*、13+16、14+15、5+10、1.5+10、174-18等优质亚基的材料,可作为优质基因源。利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)对其中的65份地方品种进行醇溶蛋白多样性分析。结果表明:电泳出现64条迁移率不同的谱带,构成65种组合,其中ω区出现的谱带最多,达17条;其次是β和γ区各16条,α区出现的谱带数最少,为15条。从每条谱带在65份材料中出现的频率看,总的变异范围为1.54%~93.85%;α、β、γ和ω4个分区多样性指数(H1)分别为0.498、0.386、0.523和0.348,表明新疆麦区小麦地方品种贮藏蛋白位点存在丰富的遗传多样性。

英文摘要:

In order to know the genetic diversity of wheat landraces in Xinjiang wheat production area and provide basic materials for improving wheat quality,subunits composition of high molecular weight gluten with a total of 236 wheat landraces were analyzed by means of SDS-PAGE. The result indicated that there were 19 alleles on Glu- 1 loci,including 3 alleles on Glu-A1 loci,7 alleles on Glu-B1 loci,9 alleles on Glu-D1 loci;And null,7 + 8,2 + 12 subunits occurred with highest frequency on each of loci, reaching 91.95 % , 85. 17% , 80.93 % respectively. There are 21 types of subunit compositions and predominant was null/7 + 8/2 + 12 with a frequency of 70.34%. Materials conferred with elite subunits of 1,2^*, 13 + 16,14 + 15,5 + 10,1.5 + 10,17 + 18 were selected and could be a source of desirable genes. Meanwhile, Gliadin of 65 wheat landraces in Xinjiang wheat production area were analyzed by A-PAGE. It was shown that 64 different spectral gliadin bands were isolated from the materials;the bands including 17 bands in ω region, 16 in β and γ regions and 15 in in region, and their frequencies ranged within 1.54% - 93.85 %. Genetic diversity index ( H' ) of α, β, γ and ω regions were 0. 498,0. 386,0. 523,0. 348, respectively. High genetic diversity of storage proteins in wheat landraees of Xinjiang wheat production region was observed.

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期刊信息
  • 《植物遗传资源学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院作物科学研究所 中国农学会
  • 主编:刘旭
  • 地址:北京中关村南大街12号
  • 邮编:100081
  • 邮箱:zwyczyxb2003@163.com/sina.com/caas.cn
  • 电话:010-82105794 82105795
  • 国际标准刊号:ISSN:1672-1810
  • 国内统一刊号:ISSN:11-4996/S
  • 邮发代号:82-643
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:10278