采用单因子实验或响应曲面法分别研究了单一酶、复合酶以及复合酶-化学药剂联合的3种调理方式下污泥的脱水性能,并探讨了典型调理条件下污泥的Zeta电位、粒度和水分组成等变化特征。研究结果表明,复合酶、十六烷基三甲基溴化铵(CTMAB)和阳离子聚丙烯酰胺WD4960(WD4960)的单一调理和联合调理均能不同程度地释放污泥颗粒中的束缚水。污泥经0.13 g·g^-1TS中温a-淀粉酶作用1 h后,再投加0.16 g·g^-1TS中性蛋白酶作用4 h,其胞外有机物(EPS)中多糖(PS)、蛋白质(PN)组分含量分别为最低值17.88、218.72 mg·g^-1VSS,但污泥絮体的Zeta电位和粒度均减小,脱水性能恶化。对于复合酶调理后的污泥,再投加0.5 g·g^-1TS CTMAB进行二次调理后,抽滤泥饼含水率降至64.66%;当投加9.64 g·kg^-1TS的WD4960进行二次调理时,污泥的毛细吸水时间(CST)为最小值16.3 s。其中,CTMAB通过电中和、改变污泥絮体的亲疏水性等作用改善复合酶调理后污泥的脱水性能,而WD4960通过电中和、粘附架桥的絮凝作用,但后者释放的束缚水较少。
Using a single factor experiment and response surface methodology( RSM),the dewaterability of activated sludge( AS) was studied under three different forms of conditioning: single enzyme,complex enzymes,and complex enzymes combined with chemicals( cetyl trimethyl ammonium bromide/cationic polyacrylamideWD4960,CTMAB/WD4960). Moreover,the variations of the zeta potential,particle size,and moisture distribution for AS samples,before and after typical conditioning treatments,were also determined. The results showed that varying proportions of bound water was released from AS conditioned in the four ways just mentioned. When AS was conditioned with complex enzymes of 0. 13 g·g^-1TS a-amylase for 1 h and 0. 16 g·g^-1TS protease for 4 h,the polysaccharide and protein contents in the extracellular polymeric substances reached their minimum values of 17. 88 and 218. 72 mg·g^-1VSS respectively. However,the zeta potential and size of AS flocs both decreased; thus the AS dewaterability deteriorated accordingly. The AS dewaterability could be improved by a combination of the above complex enzymes,with CTMAB or WD4960. The moisture content of vacuum-filtered sludge cake of AS re-conditioned using 0. 5 g·g^-1TS CTMAB,was reduced to 64. 66%. The capillary suction time for AS re-conditioned with 9. 46 g·kg^-1TS WD4960,reached its minimum value of 16. 3 s.This phenomenon implied that subsequent CTMAB dosing could ameliorate the dewaterability of AS flocs pretreated with complex enzymes,by neutralizing their negative surface charge and changing their hydrophilicity/hydrophobicity. In contrast,WD4960 functioned by neutralizing surface charges and adhesion-bridging,which ledto less bound water release from complex-enzyme treated AS,compared to follow-up treatment with CTMAB.