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蝗总科6种蝗虫6个种群的遗传分化
  • ISSN号:0253-2395
  • 期刊名称:《山西大学学报:自然科学版》
  • 时间:0
  • 分类:Q348[生物学—遗传学]
  • 作者机构:[1]山西大学生命科学与技术学院,山西太原030006
  • 相关基金:国家自然科学基金(305702479,30470219);山西省自然科学基金(200601107)
中文摘要:

采用水平淀粉凝胶电泳技术研究东亚飞蝗、大垫尖翅蝗、中华稻蝗、中华蚱蜢、亚洲小车蝗和黄胫小车蝗6个种的种群遗传结构及其分化.在所检测的13个基因座位(Ak、Ck、G3pd、Gpi、Hk-1、Hk-2、Idh-1、Idh-2、Ldh、Mdh-1、Mdh-2、Mdph、Pgm)中,有2个基因座位(G3pdh和Mdh-2)在6个种群中均为单态(0.95标准);5个基因座位(Gpi、Hk-1、Hk-2、Mdph和Pgm)在6个种群中均为多态;其余的基因座位至少在一个种群内有两个以上的等位基因.除Gpi和Mdh-1在多数种群符合哈-温(H-W)平衡期望值,其余大多数基因座位的基因型频率显著偏离哈-温(H-W)平衡(P〈0.05).从A、P、Ho和He值可知,中华蚱蜢的遗传多样性最低,其次是亚洲小车蝗和黄胫小车蝗,而大垫尖翅蝗、东亚飞蝗和中华稻蝗遗传多样性均较高.根据Roger's遗传距离用非加权算术平均法(UPGMA)进行的聚类分析符合传统形态学和细胞学研究结果.

英文摘要:

The genetic structure and differentiation of 6 populations of 6 locust species (Locusta migratoria manilensis ,Epacromius coerulipes , Oxya chinensis , Acrida cinerea , Oedaleus asiaticus and O. infernalis ) in three families were analyzed using horizontal starch gel electrophoresis. Among 13 loci,G3pdh and Mdh-2 were monomorphic across all 6 populations (0.95 criterion) ,whereas more than one alleles were present at the rest loci in at least one population. Five loci (Gpi,Hk-1 ,Hk-2,Mdph and Pgm) were highly polymorphic in all six populations (0.95 criterion) ,each with at least two alleles. The genotype frequency at most loci significantly deviated from the Hardy-Weinberg equilibrium except for Gpi and Mdh-1 in most samples (P〈0.05). The data of A,P,Ho and He of six species suggested that low genetic polymorphism was observed in Acrida chinensis population (A, =2.1 ,P=42.6% ,Ho=0. 165,He=0. 191). O. asiaticus and O. infernalis second,whereas L. migratoria manilensis,E, coerulipes and O. chinensis possess higher genetic polymorphism at 13 allozyme loci. The result of cluster analysis by using unweighted pair-group method with arithmetic averaging (UPGMA) based on Roger's genetic distance were consistent with the results obtained from karyotypie analyses. It is suggested that the allozyme analysis is an useful molecular marker for phylogenetic reconstruction.

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期刊信息
  • 《山西大学学报:自然科学版》
  • 北大核心期刊(2011版)
  • 主管单位:山西省教育厅
  • 主办单位:山西大学
  • 主编:杨斌盛
  • 地址:太原市坞城路92号
  • 邮编:030006
  • 邮箱:xbbjb@sxu.edu.cn
  • 电话:0351-7010455
  • 国际标准刊号:ISSN:0253-2395
  • 国内统一刊号:ISSN:14-1105/N
  • 邮发代号:22-42
  • 获奖情况:
  • 边疆七年获山西省一级期刊荣誉(1993-1999)
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国动物学记录,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:5651