中文摘要：

一般研究内生真菌的方法都无法预测内生真菌次级代谢产物的类型,本实验以Ⅰ型聚酮合成酶（polyketide synthase,PKS）基因为探针,野生海芦笋（Salicornia bigelovii Torr.）为原料,筛选出菌株Salicorn 3,并对其发酵产物中的化合物进行分离鉴定。结果表明：经过ITS1-5.8S-ITS4 rDNA同源序列鉴定的菌株Salicorn 3为圆弧青霉（Penicillium cyclopium）,通过系统发育树分析并结合文献数据比较,表明该菌株具有产生聚酮类化合物的潜力。通过常压硅胶柱层析和Sephadex LH-20柱层析从其发酵产物中分离得到两个单体化合物,采用核磁共振和电喷雾质谱鉴定其化学结构分别为二酮哌嗪生物碱和脑苷脂C。

英文摘要：

In this study, we aimed to predict the type of secondary metabolites of the endophyte Salicorn 3 isolated from wild Salicornia bigelovii Torr. by using the type Ⅰ polyketide synthase（PKS I） gene as a probe. As a result, two polyketide compounds were isolated from the fermentation product of Salicorn 3. Based on the ITS1-5.8S-ITS4 rDNA sequence analysis, the strain Salicorn 3 was identified as Penicillium cyclopium. The isolate was found to have the potential to produce polyketide compounds by phylogenetic analysis and comparing the data with the reference. Two pure compounds were obtained from the fermentation product of Salicorn 3 using open silica gel column chromatography and Sephadex LH-20, and their chemical structures were elucidated as（1） ditryptophenaline and（2） cerebroside C by nuclear magnetic resonance（NMR） and electrospray ionization mass spectrometry（ESI-MS）.