对一株红树林来源的产油脂真菌PJx-29发酵产物中的抗氧化成分进行分离和鉴定。利用溶剂萃取、硅胶柱色谱及制备HPLC等分离手段从发酵产物中分离得到2个甘油酯类化合物和其他5个化合物,通过理化性质分析及渡谱学方法鉴定其化学结构分别为:十五碳单甘油酯,9”,12”一十七碳二烯酸,十六烷基甘油酯,6-戊基-仪-吡喃酮,4,6-二羟基-9,10-二甲基苯乙酮,双(8—8’-7羟基-4-甲氧基-5-甲基香豆素),对羟基苯甲酸,6-氨基苯甲醛,并以DPPH法评价了化合物的抗氧化活性,结果表明化合物1,化合物2和化合物6具有较强的抗氧化活性,其Ic50分别为:(11.38±0.61)、(9.33±0.80)和(11.12±1.01)μg/mL。
The antioxidant components duction fungus PJX -29 derived from Ocean were isolated and identified from the fermentation product of the Oil pro- Mangroves. Two glyceride compounds and other five compounds were iso- lated with solvent extraction, silica gel column chromatography and preparative HPLC and their structures were identi- fied as monoglycerides ,9", 12" - Heptadecadienoic acid, 1 - (hydroxymethyl) - 2 - [ ( 1 - oxohexadecyl) oxy ] ethyl ester,6 - Pentyl - ct - pyrone ,4,6 - dihydroxy - 9,10 - dimethylacetophenone, bis [ 8 - 8' - (7 - hydroxy - 4 - me- thoxy -5 -methylcoumarin) ] , Hydroxybenzoic acid ,6 -Aminobenzaldehyde by physicochemical properties and spec- tral analyses, respectively. The antioxidant activity of the compounds were evaluated by DPPH method, the result showed that compounds 1,2 and 6 have potential antioxidant activity, and their ICs0 are ( 11.38 ± 0.61 ), (9.33 ± 0.80) and ( 11.12 ± 1.01 ) μg/mL,respectively.